1h81: Difference between revisions

Polyamine oxidase (PAO) carries out the FAD-dependent oxidation of the, secondary amino groups of spermidine and spermine, a key reaction in the, polyamine catabolism. The active site of PAO consists of a 30 A long, U-shaped catalytic tunnel, whose innermost part is located in front of the, flavin ring. To provide insight into the PAO substrate specificity and, amine oxidation mechanism, we have investigated the crystal structure of, maize PAO in the reduced state and in complex with three different, inhibitors, guazatine, 1,8-diaminooctane, and, N(1)-ethyl-N(11)-[(cycloheptyl)methyl]-4,8-diazaundecane (CHENSpm). In the, reduced state, the conformation of the isoalloxazine ring and the, surrounding residues is identical to that of the oxidized enzyme. Only, Lys300 moves away from the flavin to compensate for the change in cofactor, protonation occurring upon reduction. The structure of the PAO.inhibitor, complexes reveals an exact match between the inhibitors and the PAO, catalytic tunnel. Inhibitor binding does not involve any protein, conformational change. Such lock-and-key binding occurs also in the, complex with CHENSpm, which forms a covalent adduct with the flavin N5, atom. Comparison of the enzyme complexes hints at an "out-of-register", mechanism of inhibition, in which the inhibitor secondary amino groups are, not properly aligned with respect to the flavin to allow oxidation. Except, for the Glu62-Glu170 pair, no negatively charged residues are involved in, the recognition of substrate and inhibitor amino groups, which is in, contrast to other polyamine binding proteins. This feature may be, exploited in the design of drugs specifically targeting PAO.

1H81 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays] with NAG and FAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polyamine_oxidase Polyamine oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.3.11 1.5.3.11] Structure known Active Sites: FAA, FAB and FAC. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H81 OCA].  

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