1r9j: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1r9j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_mexicana_mexicana Leishmania mexicana mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9J FirstGlance]. <br> | <table><tr><td colspan='2'>[[1r9j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_mexicana_mexicana Leishmania mexicana mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9J FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r9j RCSB], [http://www.ebi.ac.uk/pdbsum/1r9j PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r9j RCSB], [http://www.ebi.ac.uk/pdbsum/1r9j PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Leishmania mexicana mexicana]] | [[Category: Leishmania mexicana mexicana]] | ||
[[Category: Transketolase]] | [[Category: Transketolase]] | ||
[[Category: Barrett, M P | [[Category: Barrett, M P]] | ||
[[Category: Cazzulo, J J | [[Category: Cazzulo, J J]] | ||
[[Category: Lindqvist, Y | [[Category: Lindqvist, Y]] | ||
[[Category: Mauger, D A | [[Category: Mauger, D A]] | ||
[[Category: Veitch, N J | [[Category: Veitch, N J]] | ||
[[Category: | [[Category: Domain]] | ||
[[Category: Each of the alpha/beta type]] | [[Category: Each of the alpha/beta type]] | ||
[[Category: Thiamine diphosphate binding domain]] | [[Category: Thiamine diphosphate binding domain]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 10:41, 6 January 2015
Transketolase from Leishmania mexicanaTransketolase from Leishmania mexicana
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedTransketolase has been characterized in Leishmania mexicana. A gene encoding this enzyme was identified and cloned. The gene was expressed in Escherichia coli and the protein was purified and characterized. An apparent K(m) of 2.75 mM for ribose 5-phosphate was determined. X-ray crystallography was used to determine the three-dimensional structure of the enzyme to a resolution of 2.2 A (1 A identical with 0.1 nm). The C-terminus of the protein contains a type-1 peroxisome-targeting signal, suggestive of a possible glycosomal subcellular localization. Subcellular localization experiments performed with promastigote forms of the parasite revealed that the protein was predominantly cytosolic, although a significant component of the total activity was associated with the glycosomes. Transketolase is thus the first enzyme of the nonoxidative branch of the pentose phosphate pathway whose presence has been demonstrated in a peroxisome-like organelle. Transketolase from Leishmania mexicana has a dual subcellular localization.,Veitch NJ, Maugeri DA, Cazzulo JJ, Lindqvist Y, Barrett MP Biochem J. 2004 Sep 1;382(Pt 2):759-67. PMID:15149284[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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