1mme: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1mme]] is a 4 chain structure. The May 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Self-splicing RNA'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_5 10.2210/rcsb_pdb/mom_2005_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MME FirstGlance]. <br> | <table><tr><td colspan='2'>[[1mme]] is a 4 chain structure. The May 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Self-splicing RNA'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_5 10.2210/rcsb_pdb/mom_2005_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MME FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB], [http://www.ebi.ac.uk/pdbsum/1mme PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mme OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mme RCSB], [http://www.ebi.ac.uk/pdbsum/1mme PDBsum]</span></td></tr> | ||
<table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: Self-splicing RNA]] | [[Category: Self-splicing RNA]] | ||
[[Category: Finch, J T | [[Category: Finch, J T]] | ||
[[Category: Klug, A | [[Category: Klug, A]] | ||
[[Category: Scott, W G | [[Category: Scott, W G]] | ||
[[Category: Catalytic rna]] | [[Category: Catalytic rna]] | ||
[[Category: Loop]] | [[Category: Loop]] | ||
[[Category: Ribozyme]] | [[Category: Ribozyme]] | ||
[[Category: Rna hammerhead ribozyme]] | [[Category: Rna hammerhead ribozyme]] | ||
Revision as of 20:04, 5 January 2015
THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGETHE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE
Structural highlights
Publication Abstract from PubMedWe have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage. The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage.,Scott WG, Finch JT, Klug A Cell. 1995 Jun 30;81(7):991-1002. PMID:7541315[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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