4mfi: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4mfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MFI FirstGlance]. <br> | <table><tr><td colspan='2'>[[4mfi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MFI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MFI FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mfi RCSB], [http://www.ebi.ac.uk/pdbsum/4mfi PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mfi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mfi RCSB], [http://www.ebi.ac.uk/pdbsum/4mfi PDBsum]</span></td></tr> | ||
<table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bartlam, M | [[Category: Bartlam, M]] | ||
[[Category: Jiang, D | [[Category: Jiang, D]] | ||
[[Category: Rao, Z | [[Category: Rao, Z]] | ||
[[Category: Abc transporter substrate-binding protein]] | [[Category: Abc transporter substrate-binding protein]] | ||
[[Category: Alpha/beta domain]] | [[Category: Alpha/beta domain]] | ||
[[Category: Carbohydrate/sugar binding]] | [[Category: Carbohydrate/sugar binding]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||
Revision as of 15:46, 5 January 2015
Crystal structure of Mycobacterium tuberculosis UgpBCrystal structure of Mycobacterium tuberculosis UgpB
Structural highlights
Publication Abstract from PubMedTuberculosis (TB), caused by Mycobacterium tuberculosis, is one of the most devastating human diseases, and is responsible for ~ 2 million deaths worldwide each year. The nutritional requirements for the growth of mycobacteria have been extensively studied since the discovery of M. tuberculosis, but the essential nutrients for M. tuberculosis inside the human host and the identity of the corresponding transporters remain unknown. The UgpABCE transporter of M. tuberculosis is one of five putative permeases for carbohydrate uptake, and is genetically predicted to be an sn-glycerol 3-phosphate importer. We have determined the 1.5-A crystal structure of M. tuberculosis UgpB, which has been reported to be a promising vaccine candidate against TB. M. tuberculosis UgpB showed no detectable binding activity for sn-glycerol 3-phosphate by isothermal titration calorimetry, but instead showed a preference for glycerophosphocholine (GPC). M. tuberculosis UgpB largely resembles its Escherichia coli homolog, but with the critical Trp169 in the substrate-binding site of E. coli UgpB replaced by Leu205. Mutation of Leu205 abolishes GPC binding, suggesting that Leu205 is a determinant of GPC binding. The work reported here not only contributes to our understanding of the carbon and phosphate sources utilized by M. tuberculosis inside the human host, but will also promote improvements in TB chemotherapy. Database: Structural data are available in the Protein Data Bank database under the accession number PDB 4MFI. Structural analysis of Mycobacterium tuberculosis ATP-binding cassette transporter subunit UgpB reveals specificity for glycerophosphocholine.,Jiang D, Zhang Q, Zheng Q, Zhou H, Jin J, Zhou W, Bartlam M, Rao Z FEBS J. 2014 Jan;281(1):331-41. doi: 10.1111/febs.12600. Epub 2013 Dec 2. PMID:24299297[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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