2hcd: Difference between revisions

Revision as of 18:15, 20 March 2008

, resolution 2.6Å

Ligands:

Coordinates:

Crystal structure of the ligand binding domain of the Vitamin D nuclear receptor in complex with Gemini and a coactivator peptide

OverviewOverview

The crystal structure of the ligand binding domain (LBD) of the wild-type Vitamin D receptor (VDR) of zebrafish bound to Gemini, a synthetic agonist ligand with two identical side chains branching at carbon 20 reveals a ligand-dependent structural rearrangement of the ligand binding pocket (LBP). The rotation of a Leu side chain opens the access to a channel that can accommodate the second side chain of the ligand. The 25% increase of the LBP's volume does not alter the essential agonist features of VDR. The possibility to adapt the LBP to novel ligands with different chemistry and/or structure opens new perspectives in the design of more specifically targeted ligands.

About this StructureAbout this Structure

2HCD is a Protein complex structure of sequences from Danio rerio. Full crystallographic information is available from OCA.

ReferenceReference

Adaptability of the Vitamin D nuclear receptor to the synthetic ligand Gemini: remodelling the LBP with one side chain rotation., Ciesielski F, Rochel N, Moras D, J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):235-42. Epub 2007 Jan 10. PMID:17218092

Page seeded by OCA on Thu Mar 20 17:15:21 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2hcd.jpg|left|200px]]<br /><applet load="2hcd" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2hcd.jpg|left|200px]]
-caption="2hcd, resolution 2.6&Aring;" />
-'''Crystal structure of the ligand binding domain of the Vitamin D nuclear receptor in complex with Gemini and a coactivator peptide'''<br />
+ {{Structure
+|PDB= 2hcd |SIZE=350|CAPTION= <scene name='initialview01'>2hcd</scene>, resolution 2.6&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=BIV:21-NOR-9,10-SECOCHOLESTA-5,7,10(19)-TRIENE-1,3,25-TRIOL, 20-(4-HYDROXY-4-METHYLPENTYL)-, (1A,3B,5Z,7E)'>BIV</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of the ligand binding domain of the Vitamin D nuclear receptor in complex with Gemini and a coactivator peptide'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-HCD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio] with <scene name='pdbligand=BIV:'>BIV</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HCD OCA].
+HCD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HCD OCA].
 ==Reference==
-Adaptability of the Vitamin D nuclear receptor to the synthetic ligand Gemini: remodelling the LBP with one side chain rotation., Ciesielski F, Rochel N, Moras D, J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):235-42. Epub 2007 Jan 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17218092 17218092]
+Adaptability of the Vitamin D nuclear receptor to the synthetic ligand Gemini: remodelling the LBP with one side chain rotation., Ciesielski F, Rochel N, Moras D, J Steroid Biochem Mol Biol. 2007 Mar;103(3-5):235-42. Epub 2007 Jan 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17218092 17218092]
 [[Category: Danio rerio]]
 [[Category: Protein complex]]
@@ Line 19: / Line 28: @@
 [[Category: alpha helical sandwich]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:40:24 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:15:21 2008''