4mb4: Difference between revisions

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-{{STRUCTURE_4mb4|  PDB=4mb4  |  SCENE=  }}
+==Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4==
-===Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella complex with Nag4===
+<StructureSection load='4mb4' size='340' side='right' caption='[[4mb4]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
-{{ABSTRACT_PUBMED_24598737}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4mb4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"spirillum_marinum"_russell_1891 "spirillum marinum" russell 1891]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MB4 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hmc|4hmc]], [[4hmd|4hmd]], [[4hme|4hme]], [[4mb3|4mb3]], [[4mb5|4mb5]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">chi60 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90736 "Spirillum marinum" Russell 1891])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mb4 RCSB], [http://www.ebi.ac.uk/pdbsum/4mb4 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact.
-==About this Structure==
+Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.,Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):676-84. doi:, 10.1107/S1399004713032264. Epub 2014 Feb 15. PMID:24598737<ref>PMID:24598737</ref>
-[[4mb4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB4 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024598737</ref><references group="xtra"/><references/>
+</div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Spirillum marinum russell 1891]]
 [[Category: Chitinase]]
-[[Category: Malecki, P H.]]
+[[Category: Malecki, P H]]
-[[Category: Rypniewski, W.]]
+[[Category: Rypniewski, W]]
-[[Category: Vorgias, C E.]]
+[[Category: Vorgias, C E]]
 [[Category: Alpha/beta-barrel ig-like]]
 [[Category: Chbd]]
 [[Category: Chitin binding domain]]
-[[Category: Chitinase]]
 [[Category: Hydrolase]]
 [[Category: Hydrolaze]]