2h5a: Difference between revisions

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Revision as of 18:12, 20 March 2008

File:2h5a.gif

, resolution 1.72Å

Ligands:

and

Gene:

algC (Pseudomonas aeruginosa)

Coordinates:

save as pdb, mmCIF, xml

Complex of the enzyme PMM/PGM with xylose 1-phosphate

OverviewOverview

Two complexes of the enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) from Pseudomonas aeruginosa with a slow substrate and with an inhibitor have been characterized by X-ray crystallography. Both ligands induce an interdomain rearrangement in the enzyme that creates a highly buried active site. Comparisons with enzyme-substrate complexes show that the inhibitor xylose 1-phosphate utilizes many of the previously observed enzyme-ligand interactions. In contrast, analysis of the ribose 1-phosphate complex reveals a combination of new and conserved enzyme-ligand interactions for binding. The ability of PMM/PGM to accommodate these two pentose phosphosugars in its active site may be relevant for future efforts towards inhibitor design.

About this StructureAbout this Structure

2H5A is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

ReferenceReference

Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):722-6. Epub 2006 Jul 24. PMID:16880541

Page seeded by OCA on Thu Mar 20 17:12:47 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2h5a.gif|left|200px]]<br /><applet load="2h5a" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2h5a.gif|left|200px]]
-caption="2h5a, resolution 1.72&Aring;" />
-'''Complex of the enzyme PMM/PGM with xylose 1-phosphate'''<br />
+ {{Structure
+|PDB= 2h5a |SIZE=350|CAPTION= <scene name='initialview01'>2h5a</scene>, resolution 1.72&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=X1P:1-O-PHOSPHONO-ALPHA-D-XYLOPYRANOSE'>X1P</scene>
+|ACTIVITY=
+|GENE= algC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
+}}
+'''Complex of the enzyme PMM/PGM with xylose 1-phosphate'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-H5A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=X1P:'>X1P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5A OCA].
+H5A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H5A OCA].
 ==Reference==
-Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):722-6. Epub 2006 Jul 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16880541 16880541]
+Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):722-6. Epub 2006 Jul 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16880541 16880541]
 [[Category: Pseudomonas aeruginosa]]
 [[Category: Single protein]]
@@ Line 21: / Line 30: @@
 [[Category: inhibitor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:38:17 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:12:47 2008''