2h1e: Difference between revisions
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[[Image:2h1e.gif|left|200px]] | [[Image:2h1e.gif|left|200px]] | ||
'''Tandem chromodomains of budding yeast CHD1''' | {{Structure | ||
|PDB= 2h1e |SIZE=350|CAPTION= <scene name='initialview01'>2h1e</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= CHD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |||
}} | |||
'''Tandem chromodomains of budding yeast CHD1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2H1E is a [ | 2H1E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H1E OCA]. | ||
==Reference== | ==Reference== | ||
Molecular implications of evolutionary differences in CHD double chromodomains., Flanagan JF, Blus BJ, Kim D, Clines KL, Rastinejad F, Khorasanizadeh S, J Mol Biol. 2007 Jun 1;369(2):334-42. Epub 2007 Mar 19. PMID:[http:// | Molecular implications of evolutionary differences in CHD double chromodomains., Flanagan JF, Blus BJ, Kim D, Clines KL, Rastinejad F, Khorasanizadeh S, J Mol Biol. 2007 Jun 1;369(2):334-42. Epub 2007 Mar 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17433364 17433364] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: chd1]] | [[Category: chd1]] | ||
[[Category: chromodomain]] | [[Category: chromodomain]] | ||
[[Category: tandem | [[Category: tandem chromodomain]] | ||
[[Category: three-stranded antiparallel b-sheet]] | [[Category: three-stranded antiparallel b-sheet]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:11:24 2008'' | ||
Revision as of 18:11, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Gene: | CHD1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Tandem chromodomains of budding yeast CHD1
OverviewOverview
Double chromodomains occur in CHD proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcription regulation. Biochemical studies suggest important differences in the histone H3 tail binding of different CHD chromodomains. In human and Drosophila, CHD1 double chromodomains bind lysine 4-methylated histone H3 tail, which is a hallmark of transcriptionally active chromatin in all eukaryotes. Here, we present the crystal structure of the yeast CHD1 double chromodomains, and pinpoint their differences with that of the human CHD1 double chromodomains. The most conserved residues in these double chromodomains are the two chromoboxes that orient adjacently. Only a subset of CHD chromoboxes can form an aromatic cage for methyllysine binding, and methyllysine binding requires correctly oriented inserts. These factors preclude yeast CHD1 double chromodomains from interacting with the histone H3 tail. Despite great sequence similarity between the human CHD1 and CHD2 chromodomains, variation within an insert likely prevents CHD2 double chromodomains from binding lysine 4-methylated histone H3 tail as efficiently as in CHD1. By using the available structural and biochemical data we highlight the evolutionary specialization of CHD double chromodomains, and provide insights about their targeting capacities.
About this StructureAbout this Structure
2H1E is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Molecular implications of evolutionary differences in CHD double chromodomains., Flanagan JF, Blus BJ, Kim D, Clines KL, Rastinejad F, Khorasanizadeh S, J Mol Biol. 2007 Jun 1;369(2):334-42. Epub 2007 Mar 19. PMID:17433364
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