4ios: Difference between revisions
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==Structure of phage TP901-1 RBP (ORF49) in complex with nanobody 11.== | |||
<StructureSection load='4ios' size='340' side='right' caption='[[4ios]], [[Resolution|resolution]] 2.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ios]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_phage_tp901-1 Lactococcus phage tp901-1] and [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IOS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IOS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hem|4hem]], [[4hep|4hep]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bpp, ORF49 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35345 Lactococcus phage TP901-1])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ios FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ios OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ios RCSB], [http://www.ebi.ac.uk/pdbsum/4ios PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lactococcal phages belong to a large family of Siphoviridae and infect Lactococcus lactis, a gram-positive bacterium used in commercial dairy fermentations. These phages are believed to recognize and bind specifically to pellicle polysaccharides covering the entire bacterium. The phage TP901-1 baseplate, located at the tip of the tail, harbors 18 trimeric receptor binding proteins (RBPs) promoting adhesion to a specific lactococcal strain. Phage TP901-1 adhesion does not require major conformational changes or Ca(2+), which contrasts other lactococcal phages. Here, we produced and characterized llama nanobodies raised against the purified baseplate and the Tal protein of phage TP901-1 as tools to dissect the molecular determinants of phage TP901-1 infection. Using a set of complementary techniques, surface plasmon resonance, EM, and X-ray crystallography in a hybrid approach, we identified binders to the three components of the baseplate, analyzed their affinity for their targets, and determined their epitopes as well as their functional impact on TP901-1 phage infectivity. We determined the X-ray structures of three nanobodies in complex with the RBP. Two of them bind to the saccharide binding site of the RBP and are able to fully neutralize TP901-1 phage infectivity, even after 15 passages. These results provide clear evidence for a practical use of nanobodies in circumventing lactococcal phages viral infection in dairy fermentation. | |||
Viral infection modulation and neutralization by camelid nanobodies.,Desmyter A, Farenc C, Mahony J, Spinelli S, Bebeacua C, Blangy S, Veesler D, van Sinderen D, Cambillau C Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1371-9. doi:, 10.1073/pnas.1301336110. Epub 2013 Mar 25. PMID:23530214<ref>PMID:23530214</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactococcus phage tp901-1]] | [[Category: Lactococcus phage tp901-1]] | ||
[[Category: Lama glama]] | [[Category: Lama glama]] | ||
[[Category: Bebeacua, C | [[Category: Bebeacua, C]] | ||
[[Category: Blangy, S | [[Category: Blangy, S]] | ||
[[Category: Cambillau, C | [[Category: Cambillau, C]] | ||
[[Category: Desmyter, A | [[Category: Desmyter, A]] | ||
[[Category: Farenc, C | [[Category: Farenc, C]] | ||
[[Category: Mahony, J | [[Category: Mahony, J]] | ||
[[Category: Sinderen, D van | [[Category: Sinderen, D van]] | ||
[[Category: Spinelli, S | [[Category: Spinelli, S]] | ||
[[Category: Veesler, D | [[Category: Veesler, D]] | ||
[[Category: All beta]] | [[Category: All beta]] | ||
[[Category: Cell adhesion-immune system complex]] | [[Category: Cell adhesion-immune system complex]] | ||