4al0: Difference between revisions

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-[[Image:4al0.png|left|200px]]
+==Crystal structure of Human PS-1==
+<StructureSection load='4al0' size='340' side='right' caption='[[4al0]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4al0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AL0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AL0 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4al1|4al1]]</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-E_synthase Prostaglandin-E synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.3 5.3.99.3] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4al0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4al0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4al0 RCSB], [http://www.ebi.ac.uk/pdbsum/4al0 PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/PTGES_HUMAN PTGES_HUMAN]] Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).<ref>PMID:18682561</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Prostaglandin E (PGE) is a key mediator in inflammatory response. The main source of inducible PGE, microsomal PGE synthase-1 (mPGES-1), has emerged as an interesting drug target for treatment of pain. To support inhibitor design, we have determined the crystal structure of human mPGES-1 to 1.2 A resolution. The structure reveals three well-defined active site cavities within the membrane-spanning region in each monomer interface of the trimeric structure. An important determinant of the active site cavity is a small cytosolic domain inserted between transmembrane helices I and II. This extra domain is not observed in other structures of proteins within the MAPEG (Membrane-Associated Proteins involved in Eicosanoid and Glutathione metabolism) superfamily but is likely to be present also in microsomal GST-1 based on sequence similarity. An unexpected feature of the structure is a 16-A-deep cone-shaped cavity extending from the cytosolic side into the membrane-spanning region. We suggest a potential role for this cavity in substrate access. Based on the structure of the active site, we propose a catalytic mechanism in which serine 127 plays a key role. We have also determined the structure of mPGES-1 in complex with a glutathione-based analog, providing insight into mPGES-1 flexibility and potential for structure-based drug design.
-{{STRUCTURE_4al0|  PDB=4al0  |  SCENE=  }}
+Crystal structure of microsomal prostaglandin E2 synthase provides insight into diversity in the MAPEG superfamily.,Sjogren T, Nord J, Ek M, Johansson P, Liu G, Geschwindner S Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3806-11. doi:, 10.1073/pnas.1218504110. Epub 2013 Feb 19. PMID:23431194<ref>PMID:23431194</ref>
-===Crystal structure of Human PS-1===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-==About this Structure==
+<references/>
-[[4al0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AL0 OCA].
+__TOC__
-[[Category: Homo sapiens]]
+</StructureSection>
+[[Category: Human]]
 [[Category: Prostaglandin-E synthase]]
-[[Category: Ek, M.]]
+[[Category: Ek, M]]
-[[Category: Geschwindner, S.]]
+[[Category: Geschwindner, S]]
-[[Category: Johansson, P.]]
+[[Category: Johansson, P]]
-[[Category: Liu, G.]]
+[[Category: Liu, G]]
-[[Category: Nord, J.]]
+[[Category: Nord, J]]
-[[Category: Sjogren, T.]]
+[[Category: Sjogren, T]]
 [[Category: Isomerase]]
 [[Category: Lipid biosynthesis]]
 [[Category: Membrane protein]]