2gm4: Difference between revisions
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[[Image:2gm4.gif|left|200px]] | [[Image:2gm4.gif|left|200px]] | ||
'''An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA''' | {{Structure | ||
|PDB= 2gm4 |SIZE=350|CAPTION= <scene name='initialview01'>2gm4</scene>, resolution 3.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= tnpR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GM4 is a [ | 2GM4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GM4 OCA]. | ||
==Reference== | ==Reference== | ||
Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination., Kamtekar S, Ho RS, Cocco MJ, Li W, Wenwieser SV, Boocock MR, Grindley ND, Steitz TA, Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10642-7. Epub 2006 Jun 28. PMID:[http:// | Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination., Kamtekar S, Ho RS, Cocco MJ, Li W, Wenwieser SV, Boocock MR, Grindley ND, Steitz TA, Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10642-7. Epub 2006 Jun 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16807292 16807292] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: site specific recombination]] | [[Category: site specific recombination]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:06:18 2008'' | ||
Revision as of 18:06, 20 March 2008
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| , resolution 3.50Å | |||||||
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| Gene: | tnpR (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA
OverviewOverview
The structures of two mutants of the site-specific recombinase, gammadelta resolvase, that form activated tetramers have been determined. One, at 3.5-A resolution, forms a synaptic intermediate of resolvase that is covalently linked to two cleaved DNAs, whereas the other is of an unliganded structure determined at 2.1-A resolution. Comparisons of the four known tetrameric resolvase structures show that the subunits interact through the formation of a common core of four helices. The N-terminal halves of these helices superimpose well on each other, whereas the orientations of their C termini are more variable. The catalytic domains of resolvase in the unliganded structure are arranged asymmetrically, demonstrating that their positions can move substantially while preserving the four-helix core that forms the tetramer. These results suggest that the precleavage synaptic tetramer of gammadelta resolvase, whose structure is not known, may be formed by a similar four-helix core, but differ in the relative orientations of its catalytic and DNA-binding domains.
About this StructureAbout this Structure
2GM4 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Implications of structures of synaptic tetramers of gamma delta resolvase for the mechanism of recombination., Kamtekar S, Ho RS, Cocco MJ, Li W, Wenwieser SV, Boocock MR, Grindley ND, Steitz TA, Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10642-7. Epub 2006 Jun 28. PMID:16807292
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