3vc0: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3vc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oxyuranus_scutellatus_scutellatus Oxyuranus scutellatus scutellatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VC0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3vc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oxyuranus_scutellatus_scutellatus Oxyuranus scutellatus scutellatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VC0 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vbz|3vbz]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vbz|3vbz]]</td></tr> | ||
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vc0 RCSB], [http://www.ebi.ac.uk/pdbsum/3vc0 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vc0 RCSB], [http://www.ebi.ac.uk/pdbsum/3vc0 PDBsum]</span></td></tr> | ||
<table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Oxyuranus scutellatus scutellatus]] | [[Category: Oxyuranus scutellatus scutellatus]] | ||
[[Category: Beltramini, M | [[Category: Beltramini, M]] | ||
[[Category: Cendron, L | [[Category: Cendron, L]] | ||
[[Category: Laureto, P Polverino de | [[Category: Laureto, P Polverino de]] | ||
[[Category: Micetic, I | [[Category: Micetic, I]] | ||
[[Category: Paoli, M | [[Category: Paoli, M]] | ||
[[Category: Calcium binding]] | [[Category: Calcium binding]] | ||
[[Category: Neurotoxin]] | [[Category: Neurotoxin]] | ||
Revision as of 12:38, 4 January 2015
Crystal structure of Taipoxin beta subunit isoform 1Crystal structure of Taipoxin beta subunit isoform 1
Structural highlights
Publication Abstract from PubMedSnake pre-synaptic neurotoxins endowed with phospholipase A(2) activity are potent inducers of paralysis through the specific disruption of the neuromuscular junction pre-synaptic membrane and represent a valuable tool for investigating neuronal degeneration and recovery. They have different structural complexity and a wide range of lethal potency and enzymatic activity, although they share a similar mechanism of action. Although no correlation has been reported between neurotoxicity and enzymatic activity, toxicity increases with structural complexity and phospholipase A(2) oligomers show 10-fold lower LD(50) values compared to their monomeric counterparts. To date, no structural study has been performed on multimeric SPANs with the aim of shedding light on the correlation between structural complexity and neurotoxicity. In the present study, we investigated the structure of taipoxin, a trimeric phospholipase A(2) neurotoxin, as well as that of its subunits, by X-ray crystallography and small angle X-ray scattering analysis. We present the high-resolution structure of two isoforms of the taipoxin beta subunit, which show no neurotoxic activity but enhance the activity of the other subunits in the complex. One isoform shows no structural change that could justify the lack of activity. The other displays three point mutations in critical positions for the catalytic activity. Moreover, we designed a model for the quaternary structure of taipoxin under physiological conditions, in which the three subunits are organized into a flat holotoxin with the substrate binding sockets exposed on the same side of the complex, which suggests a role for this interface in the toxin-membrane interaction. Database The coordinates and structure factors have been deposited in the RCSB Protein Data Bank (http://www.rcsb.org) under accession numbers 3VBZ and 3VCO, corresponding to beta isoforms 1 and 2 respectively Structure digital abstract * taipoxin beta isoform 2 and taipoxin beta isoform 2 bind by x-ray crystallography (View interaction). Structural analysis of trimeric phospholipase A(2) neurotoxin from the Australian taipan snake venom.,Cendron L, Micetic I, Polverino de Laureto P, Paoli M FEBS J. 2012 Jul 6. doi: 10.1111/j.1742-4658.2012.08691.x. PMID:22776098[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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