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[[ | ==Computationally Designed Self-assembling Octahedral Cage protein, O333, Crystallized in space group R32== | ||
<StructureSection load='3vcd' size='340' side='right' caption='[[3vcd]], [[Resolution|resolution]] 2.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vcd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VCD FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3n79|3n79]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PduT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 Salmonella enterica])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vcd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vcd RCSB], [http://www.ebi.ac.uk/pdbsum/3vcd PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials. | |||
Computational design of self-assembling protein nanomaterials with atomic level accuracy.,King NP, Sheffler W, Sawaya MR, Vollmar BS, Sumida JP, Andre I, Gonen T, Yeates TO, Baker D Science. 2012 Jun 1;336(6085):1171-4. PMID:22654060<ref>PMID:22654060</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Salmonella enterica]] | [[Category: Salmonella enterica]] | ||
[[Category: Baker, D | [[Category: Baker, D]] | ||
[[Category: King, N P | [[Category: King, N P]] | ||
[[Category: Sawaya, M R | [[Category: Sawaya, M R]] | ||
[[Category: Sheffler, W | [[Category: Sheffler, W]] | ||
[[Category: Yeates, T O | [[Category: Yeates, T O]] | ||
[[Category: Electron transport]] | [[Category: Electron transport]] | ||
[[Category: Self assembling octahedral cage design]] | [[Category: Self assembling octahedral cage design]] | ||
Revision as of 12:35, 4 January 2015
Computationally Designed Self-assembling Octahedral Cage protein, O333, Crystallized in space group R32Computationally Designed Self-assembling Octahedral Cage protein, O333, Crystallized in space group R32
Structural highlights
Publication Abstract from PubMedWe describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials. Computational design of self-assembling protein nanomaterials with atomic level accuracy.,King NP, Sheffler W, Sawaya MR, Vollmar BS, Sumida JP, Andre I, Gonen T, Yeates TO, Baker D Science. 2012 Jun 1;336(6085):1171-4. PMID:22654060[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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