2gjw: Difference between revisions
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[[Image:2gjw.gif|left|200px]] | [[Image:2gjw.gif|left|200px]] | ||
'''RNA Recognition and Cleavage by an Splicing Endonuclease''' | {{Structure | ||
|PDB= 2gjw |SIZE=350|CAPTION= <scene name='initialview01'>2gjw</scene>, resolution 2.85Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/tRNA-intron_endonuclease tRNA-intron endonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.9 3.1.27.9] | |||
|GENE= endA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus]) | |||
}} | |||
'''RNA Recognition and Cleavage by an Splicing Endonuclease''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GJW is a [ | 2GJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJW OCA]. | ||
==Reference== | ==Reference== | ||
RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:[http:// | RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16690865 16690865] | ||
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: splicing endonuclease af]] | [[Category: splicing endonuclease af]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:05:35 2008'' | ||
Revision as of 18:05, 20 March 2008
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| , resolution 2.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | endA (Archaeoglobus fulgidus) | ||||||
| Activity: | tRNA-intron endonuclease, with EC number 3.1.27.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA Recognition and Cleavage by an Splicing Endonuclease
OverviewOverview
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
About this StructureAbout this Structure
2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
ReferenceReference
RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865
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