2gis: Difference between revisions
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[[Image:2gis.gif|left|200px]] | [[Image:2gis.gif|left|200px]] | ||
'''Structure of the S-adenosylmethionine riboswitch mRNA regulatory element''' | {{Structure | ||
|PDB= 2gis |SIZE=350|CAPTION= <scene name='initialview01'>2gis</scene>, resolution 2.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene> and <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Structure of the S-adenosylmethionine riboswitch mRNA regulatory element''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GIS is a [ | 2GIS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIS OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http:// | Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16810258 16810258] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Batey, R T.]] | [[Category: Batey, R T.]] | ||
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[[Category: sam]] | [[Category: sam]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:05:08 2008'' | ||
Revision as of 18:05, 20 March 2008
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| , resolution 2.90Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the S-adenosylmethionine riboswitch mRNA regulatory element
OverviewOverview
Riboswitches are cis-acting genetic regulatory elements found in the 5'-untranslated regions of messenger RNAs that control gene expression through their ability to bind small molecule metabolites directly. Regulation occurs through the interplay of two domains of the RNA: an aptamer domain that responds to intracellular metabolite concentrations and an expression platform that uses two mutually exclusive secondary structures to direct a decision-making process. In Gram-positive bacteria such as Bacillus species, riboswitches control the expression of more than 2% of all genes through their ability to respond to a diverse set of metabolites including amino acids, nucleobases and protein cofactors. Here we report the 2.9-angstroms resolution crystal structure of an S-adenosylmethionine (SAM)-responsive riboswitch from Thermoanaerobacter tengcongensis complexed with S-adenosylmethionine, an RNA element that controls the expression of several genes involved in sulphur and methionine metabolism. This RNA folds into a complex three-dimensional architecture that recognizes almost every functional group of the ligand through a combination of direct and indirect readout mechanisms. Ligand binding induces the formation of a series of tertiary interactions with one of the helices, serving as a communication link between the aptamer and expression platform domains.
About this StructureAbout this Structure
2GIS is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the S-adenosylmethionine riboswitch regulatory mRNA element., Montange RK, Batey RT, Nature. 2006 Jun 29;441(7097):1172-5. PMID:16810258
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