3vni: Difference between revisions
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[[ | ==Crystal structures of D-Psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars== | ||
<StructureSection load='3vni' size='340' side='right' caption='[[3vni]], [[Resolution|resolution]] 1.98Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vni]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_cellulolyticum Clostridium cellulolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VNI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VNI FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vnj|3vnj]], [[3vnk|3vnk]], [[3vnl|3vnl]], [[3vnm|3vnm]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ccel_0941 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1521 Clostridium cellulolyticum])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vni OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vni RCSB], [http://www.ebi.ac.uk/pdbsum/3vni PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
D-psicose 3-epimerase (DPEase) is demonstrated to be useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature. Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase. In this study, the crystal structure of the C. cellulolyticum DPEase was determined. The enzyme assembles into a tetramer and each subunit shows a (beta/alpha)(8) TIM barrel fold with a Mn(2+) metal ion in the active site. Additional crystal structures of the enzyme in complex with substrates/products (D-psicose, D-fructose, D-tagatose and D-sorbose) were also determined. From the complex structures of C. cellulolyticum DPEase with D-psicose and D-fructose, the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues. Accordingly, based on these ketohexose-bound complex structures, a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here. These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis. | |||
Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars.,Chan HC, Zhu Y, Hu Y, Ko TP, Huang CH, Ren F, Chen CC, Ma Y, Guo RT, Sun Y Protein Cell. 2012 Feb;3(2):123-31. Epub 2012 Mar 17. PMID:22426981<ref>PMID:22426981</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Clostridium cellulolyticum]] | [[Category: Clostridium cellulolyticum]] | ||
[[Category: Chan, H C | [[Category: Chan, H C]] | ||
[[Category: Chen, C C | [[Category: Chen, C C]] | ||
[[Category: Guo, R T | [[Category: Guo, R T]] | ||
[[Category: Hu, Y | [[Category: Hu, Y]] | ||
[[Category: Huang, C H | [[Category: Huang, C H]] | ||
[[Category: Ko, T P | [[Category: Ko, T P]] | ||
[[Category: Ren, F | [[Category: Ren, F]] | ||
[[Category: Sun, Y | [[Category: Sun, Y]] | ||
[[Category: Zhu, Y | [[Category: Zhu, Y]] | ||
[[Category: D-psicose 3-epimerase]] | [[Category: D-psicose 3-epimerase]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
[[Category: Ketohexose]] | [[Category: Ketohexose]] | ||