2gh7: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2gh7.gif|left|200px]] | [[Image:2gh7.gif|left|200px]] | ||
'''Epi-biotin complex with core streptavidin''' | {{Structure | ||
|PDB= 2gh7 |SIZE=350|CAPTION= <scene name='initialview01'>2gh7</scene>, resolution 1.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=BTQ:EPI-BIOTIN'>BTQ</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Epi-biotin complex with core streptavidin''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2GH7 is a [ | 2GH7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GH7 OCA]. | ||
==Reference== | ==Reference== | ||
The high-resolution structure of (+)-epi-biotin bound to streptavidin., Le Trong I, Aubert DG, Thomas NR, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):576-81. Epub 2006, May 12. PMID:[http:// | The high-resolution structure of (+)-epi-biotin bound to streptavidin., Le Trong I, Aubert DG, Thomas NR, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):576-81. Epub 2006, May 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16699183 16699183] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces avidinii]] | [[Category: Streptomyces avidinii]] | ||
| Line 21: | Line 30: | ||
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: biotin]] | [[Category: biotin]] | ||
[[Category: protein/ligand | [[Category: protein/ligand interaction]] | ||
[[Category: streptavidin]] | [[Category: streptavidin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:04:38 2008'' | ||
Revision as of 18:04, 20 March 2008
| |||||||
| , resolution 1.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Epi-biotin complex with core streptavidin
OverviewOverview
(+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral center at atom C2. This could lead to a difference in the mode of binding of (+)-epi-biotin to streptavidin, a natural protein receptor for (+)-biotin. Diffraction data were collected to a maximum of 0.85 Angstrom resolution for structural analysis of the complex of streptavidin with a sample of (+)-epi-biotin and refinement was carried out at both 1.0 and 0.85 Angstrom resolution. The structure determination shows a superposition of two ligands in the binding site, (+)-biotin and (+)-epi-biotin. The molecules overlap in the model for the complex except for the position of S1 in the tetrahydrothiophene ring. Differences in the conformation of the ring permits binding of each molecule to streptavidin with little observable difference in the protein structures at this high resolution.
About this StructureAbout this Structure
2GH7 is a Single protein structure of sequence from Streptomyces avidinii. Full crystallographic information is available from OCA.
ReferenceReference
The high-resolution structure of (+)-epi-biotin bound to streptavidin., Le Trong I, Aubert DG, Thomas NR, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):576-81. Epub 2006, May 12. PMID:16699183
Page seeded by OCA on Thu Mar 20 17:04:38 2008