2gef: Difference between revisions
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[[Image:2gef.gif|left|200px]] | [[Image:2gef.gif|left|200px]] | ||
'''Crystal structure of a Novel viral protease with a serine/lysine catalytic dyad mechanism''' | {{Structure | ||
|PDB= 2gef |SIZE=350|CAPTION= <scene name='initialview01'>2gef</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= birnaviridae ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=311176 Blotched snakehead virus]) | |||
}} | |||
'''Crystal structure of a Novel viral protease with a serine/lysine catalytic dyad mechanism''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2GEF is a [ | 2GEF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Blotched_snakehead_virus Blotched snakehead virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEF OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of a novel viral protease with a serine/lysine catalytic dyad mechanism., Feldman AR, Lee J, Delmas B, Paetzel M, J Mol Biol. 2006 May 19;358(5):1378-89. Epub 2006 Mar 6. PMID:[http:// | Crystal structure of a novel viral protease with a serine/lysine catalytic dyad mechanism., Feldman AR, Lee J, Delmas B, Paetzel M, J Mol Biol. 2006 May 19;358(5):1378-89. Epub 2006 Mar 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16584747 16584747] | ||
[[Category: Blotched snakehead virus]] | [[Category: Blotched snakehead virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: serine/lysine dyad mechamism]] | [[Category: serine/lysine dyad mechamism]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:03:44 2008'' | ||
Revision as of 18:03, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Gene: | birnaviridae (Blotched snakehead virus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a Novel viral protease with a serine/lysine catalytic dyad mechanism
OverviewOverview
The blotched snakehead virus (BSNV), an aquatic birnavirus, encodes a polyprotein (NH2-pVP2-X-VP4-VP3-COOH) that is processed through the proteolytic activity of its own protease (VP4) to liberate itself and the viral proteins pVP2, X and VP3. The protein pVP2 is further processed by VP4 to give rise to the capsid protein VP2 and four structural peptides. We report here the crystal structure of a VP4 protease from BSNV, which displays a catalytic serine/lysine dyad in its active site. This is the first crystal structure of a birnavirus protease and the first crystal structure of a viral protease that utilizes a lysine general base in its catalytic mechanism. The topology of the VP4 substrate binding site is consistent with the enzymes substrate specificity and a nucleophilic attack from the si-face of the substrates scissile bond. Despite low levels of sequence identity, VP4 shows similarities in its active site to other characterized Ser/Lys proteases such as signal peptidase, LexA protease and Lon protease. Together, the structure of VP4 provides insights into the mechanism of a recently characterized clan of serine proteases that utilize a lysine general base and reveals the structure of potential targets for antiviral therapy, especially for other related and economically important viruses, such as infectious bursal disease virus in poultry and infectious pancreatic necrosis virus in aquaculture.
About this StructureAbout this Structure
2GEF is a Single protein structure of sequence from Blotched snakehead virus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a novel viral protease with a serine/lysine catalytic dyad mechanism., Feldman AR, Lee J, Delmas B, Paetzel M, J Mol Biol. 2006 May 19;358(5):1378-89. Epub 2006 Mar 6. PMID:16584747
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