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==Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.== | |||
<StructureSection load='2map' size='340' side='right' caption='[[2map]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2map]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecod1 Ecod1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MAP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MAP FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2mao|2mao]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoE, EcDH1_1095, ECDH1ME8569_2500 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=536056 ECOD1])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2map FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2map OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2map RCSB], [http://www.ebi.ac.uk/pdbsum/2map PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors. | |||
Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660<ref>PMID:24531660</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Allain, F H-T | |||
[[Category: Campagne, S | ==See Also== | ||
[[Category: Vorholt, J A | *[[Sigma factor|Sigma factor]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Ecod1]] | |||
[[Category: Allain, F H-T]] | |||
[[Category: Campagne, S]] | |||
[[Category: Vorholt, J A]] | |||
[[Category: 10 element recognition]] | |||
[[Category: Ecf sigma factor]] | [[Category: Ecf sigma factor]] | ||
[[Category: Protein-ssdna complex]] | [[Category: Protein-ssdna complex]] | ||
[[Category: Transcription-dna complex]] | [[Category: Transcription-dna complex]] | ||
Revision as of 00:52, 4 January 2015
Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.
Structural highlights
Publication Abstract from PubMedBacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors. Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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