3mko: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3mko]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lymphocytic_choriomeningitis_virus Lymphocytic choriomeningitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MKO FirstGlance]. <br> | <table><tr><td colspan='2'>[[3mko]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lymphocytic_choriomeningitis_virus Lymphocytic choriomeningitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MKO FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lcmv-GP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11623 Lymphocytic choriomeningitis virus])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lcmv-GP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11623 Lymphocytic choriomeningitis virus])</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mko OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mko RCSB], [http://www.ebi.ac.uk/pdbsum/3mko PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mko OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mko RCSB], [http://www.ebi.ac.uk/pdbsum/3mko PDBsum]</span></td></tr> | ||
<table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation.,Igonet S, Vaney MC, Vonhrein C, Bricogne G, Stura EA, Hengartner H, Eschli B, Rey FA Proc Natl Acad Sci U S A. 2011 Nov 28. PMID:22123988<ref>PMID:22123988</ref> | X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation.,Igonet S, Vaney MC, Vonhrein C, Bricogne G, Stura EA, Hengartner H, Eschli B, Rey FA Proc Natl Acad Sci U S A. 2011 Nov 28. PMID:22123988<ref>PMID:22123988</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
== References == | == References == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Lymphocytic choriomeningitis virus]] | [[Category: Lymphocytic choriomeningitis virus]] | ||
[[Category: Igonet, S | [[Category: Igonet, S]] | ||
[[Category: Rey, F A | [[Category: Rey, F A]] | ||
[[Category: Vaney, M C | [[Category: Vaney, M C]] | ||
[[Category: Trimeric coiled-coil]] | [[Category: Trimeric coiled-coil]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 00:11, 4 January 2015
Crystal Structure of the Lymphocytic Choriomeningitis Virus Membrane Fusion Glycoprotein GP2 in its Postfusion ConformationCrystal Structure of the Lymphocytic Choriomeningitis Virus Membrane Fusion Glycoprotein GP2 in its Postfusion Conformation
Structural highlights
Publication Abstract from PubMedArenaviruses are important agents of zoonotic disease worldwide. The virions expose a tripartite envelope glycoprotein complex at their surface, formed by the glycoprotein subunits GP1, GP2 and the stable signal peptide. This complex is responsible for binding to target cells and for the subsequent fusion of viral and host-cell membranes for entry. During this process, the acidic environment of the endosome triggers a fusogenic conformational change in the transmembrane GP2 subunit of the complex. We report here the crystal structure of the recombinant GP2 ectodomain of the lymphocytic choriomeningitis virus, the arenavirus type species, at 1.8-A resolution. The structure shows the characteristic trimeric coiled coil present in class I viral fusion proteins, with a central stutter that allows a close structural alignment with most of the available structures of class I and III viral fusion proteins. The structure further shows a number of intrachain salt bridges stabilizing the postfusion hairpin conformation, one of which involves an aspartic acid that appears released from a critical interaction with the stable signal peptide upon low pH activation. X-ray structure of the arenavirus glycoprotein GP2 in its postfusion hairpin conformation.,Igonet S, Vaney MC, Vonhrein C, Bricogne G, Stura EA, Hengartner H, Eschli B, Rey FA Proc Natl Acad Sci U S A. 2011 Nov 28. PMID:22123988[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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