Sandbox Reserved 958: Difference between revisions
No edit summary |
No edit summary |
||
| Line 20: | Line 20: | ||
== DNA binding == | == DNA binding == | ||
The recognition of the viral DNA is an important step for the '''3' processing''' reaction. One of the key elements which performs the specific binding between the integrase and the cognate viral DNA is the <scene name='60/604477/Alpha4_helix/1'>alpha4 helix</scene> that is situated at the catalytic core surface<ref name="Hobaika">PMID:19808934</ref>. | The recognition of the viral DNA is an important step for the '''3' processing''' reaction. One of the key elements which performs the specific binding between the integrase and the cognate viral DNA is the <scene name='60/604477/Alpha4_helix/1'>alpha4 helix</scene> that is situated at the catalytic core surface<ref name="Hobaika">PMID:19808934</ref>. | ||
In order to have a tight binding, the integrase-DNA interaction requires optimal number of contacts such as hydrogen bonds, van der Waals forces and ionic interactions between DNA elements (bases, phosphates and sugars) and amino acid chains. Among the alpha4 helix residues,<scene name='60/604477/Lys_residues_alpha4_helix/1'>Lys156 and Lys159</scene> have a main role in the formation of bidentate hydrogen bonds which are higher specific than single hydrogen bonds<ref name="Hobaika">PMID:19808934</ref>. | |||
== Function == | == Function == | ||