1ir6: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1ir6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IR6 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1ir6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IR6 FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>< | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ir6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ir6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ir6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ir6 PDBsum], [http://www.topsan.org/Proteins/RSGI/1ir6 TOPSAN]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ir6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ir6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ir6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ir6 PDBsum], [http://www.topsan.org/Proteins/RSGI/1ir6 TOPSAN]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Fukuyama, K | [[Category: Fukuyama, K]] | ||
[[Category: Kakuta, Y | [[Category: Kakuta, Y]] | ||
[[Category: Masui, R | [[Category: Masui, R]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: Yamagata, A | [[Category: Yamagata, A]] | ||
[[Category: Dna recombination]] | [[Category: Dna recombination]] | ||
[[Category: Dna repair]] | [[Category: Dna repair]] | ||
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[[Category: Manganese]] | [[Category: Manganese]] | ||
[[Category: Nuclease]] | [[Category: Nuclease]] | ||
[[Category: Rsgi]] | [[Category: Rsgi]] | ||
[[Category: Single-stranded dna]] | [[Category: Single-stranded dna]] | ||
[[Category: Two domains interconnected by alpha-helix]] | [[Category: Two domains interconnected by alpha-helix]] | ||
Revision as of 12:40, 2 January 2015
Crystal structure of exonuclease RecJ bound to manganeseCrystal structure of exonuclease RecJ bound to manganese
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedRecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions in DNA repair and recombination systems. We determined the crystal structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and is coordinated by conserved residues characteristic of a family of phosphoesterases that includes RecJ proteins. The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA. The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity.,Yamagata A, Kakuta Y, Masui R, Fukuyama K Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5908-12. Epub 2002 Apr 23. PMID:11972066[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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