1i3v: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1i3v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I3V FirstGlance]. <br> | <table><tr><td colspan='2'>[[1i3v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I3V FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i3u|1i3u]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i3u|1i3u]]</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i3v RCSB], [http://www.ebi.ac.uk/pdbsum/1i3v PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i3v RCSB], [http://www.ebi.ac.uk/pdbsum/1i3v PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Lama glama]] | [[Category: Lama glama]] | ||
[[Category: Cambillau, C | [[Category: Cambillau, C]] | ||
[[Category: Frenken, L | [[Category: Frenken, L]] | ||
[[Category: Spinelli, S | [[Category: Spinelli, S]] | ||
[[Category: Tegoni, M | [[Category: Tegoni, M]] | ||
[[Category: Vliet, C van | [[Category: Vliet, C van]] | ||
[[Category: Antibody]] | [[Category: Antibody]] | ||
[[Category: Domain vhh]] | [[Category: Domain vhh]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
[[Category: Lama]] | [[Category: Lama]] | ||
Revision as of 12:39, 2 January 2015
THREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDEDTHREE-DIMENSIONAL STRUCTURE OF A LAMA VHH DOMAIN UNLIGANDED
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCamelids, camels and llamas, have a unique immune system able to produce heavy-chain only antibodies. Their VH domains (VHHs) are the smallest binding units produced by immune systems, and therefore suitable for biotechnological applications through heterologous expression. The recognition of protein antigens by these VHHs is rather well documented, while less is known about the VHH/hapten interactions. The recently reported X-ray structure of a VHH in complex with a copper-containing azo-dye settled the ability of VHH to recognize haptens by forming a cavity between the three complementarity-determining regions (CDR). Here we report the structures of a VHH (VHH A52) free or complexed with an azo-dye, RR1, without metal ion. The structure of the complex illustrates the involvement of CDR2, CDR3 and a framework residue in a lateral interaction with the hapten. Such a lateral combining site is comparable to that found in classical antibodies, although in the absence of the VL. Lateral recognition of a dye hapten by a llama VHH domain.,Spinelli S, Tegoni M, Frenken L, van Vliet C, Cambillau C J Mol Biol. 2001 Aug 3;311(1):123-9. PMID:11469862[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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