1w47: Difference between revisions
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==Overview== | ==Overview== | ||
Many viruses package their genome into preformed capsids using packaging, motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA, bacteriophage phi12, located at the vertices of the icosahedral capsid, is, such a packaging motor. We have captured crystallographic structures of P4, for all the key points along the catalytic pathway, including apo, substrate analog bound, and product bound. Substrate and product binding, have been observed as both binary complexes and ternary complexes with, divalent cations. These structures reveal large movements of the putative, RNA binding loop, which are coupled with nucleotide binding and, hydrolysis, indicating how ATP hydrolysis drives RNA translocation through, cooperative conformational changes. Two distinct conformations of .. | Many viruses package their genome into preformed capsids using packaging, motors powered by the hydrolysis of ATP. The hexameric ATPase P4 of dsRNA, bacteriophage phi12, located at the vertices of the icosahedral capsid, is, such a packaging motor. We have captured crystallographic structures of P4, for all the key points along the catalytic pathway, including apo, substrate analog bound, and product bound. Substrate and product binding, have been observed as both binary complexes and ternary complexes with, divalent cations. These structures reveal large movements of the putative, RNA binding loop, which are coupled with nucleotide binding and, hydrolysis, indicating how ATP hydrolysis drives RNA translocation through, cooperative conformational changes. Two distinct conformations of bound, nucleotide triphosphate suggest how hydrolysis is activated by RNA, binding. This provides a model for chemomechanical coupling for a, prototype of the large family of hexameric helicases and oligonucleotide, translocating enzymes. | ||
==About this Structure== | ==About this Structure== | ||
1W47 is a | 1W47 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi12 Pseudomonas phage phi12] with MN and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W47 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: packaging atpase]] | [[Category: packaging atpase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:44:49 2007'' | ||