Practical Guide to Homology Modeling: Difference between revisions
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If empirical structures exist, see sections below for guidance on how to explore them. If they are satisfactory, then you don't need a homology model. | If empirical structures exist, see sections below for guidance on how to explore them. If they are satisfactory, then you don't need a homology model. | ||
==== Advanced search for empirical models (RCSB PDB) ==== | |||
This method takes more time but gives you more information. It will find empirical structures that have sequence similarity to the query. Such hits enable a high-quality homology model. | |||
For example, if your query is calmodulin from the lancelet fish (Q9UB37, CALM2_BRALA), zero empirical structures are listed at UniProt. However, the query is 97% sequence identical to human calmodulin (P62158 CALM_HUMAN) and calmodulins from other taxa, for which there are numerous full-length empirical structures. A very high quality homology model can be constructed. | |||
Advanced search procedure: | |||
== References == | == References == | ||
<references/> | <references/> | ||