2fl1: Difference between revisions

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Revision as of 17:53, 20 March 2008

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, resolution 2.4Å

Ligands:

Coordinates:

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Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution

OverviewOverview

The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.

About this StructureAbout this Structure

2FL1 is a Single protein structure of sequence from Zoanthus sp.. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946

Page seeded by OCA on Thu Mar 20 16:53:39 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:2fl1.jpg|left|200px]]<br /><applet load="2fl1" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2fl1.jpg|left|200px]]
-caption="2fl1, resolution 2.4&Aring;" />
-'''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''<br />
+ {{Structure
+|PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-FL1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL1 OCA].
+FL1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zoanthus_sp. Zoanthus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FL1 OCA].
 ==Reference==
-Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16627946 16627946]
+Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16627946 16627946]
 [[Category: Single protein]]
 [[Category: Zoanthus sp.]]
@@ Line 33: / Line 42: @@
 [[Category: zrfp574]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:22:28 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:53:39 2008''