2ffl: Difference between revisions

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Revision as of 17:51, 20 March 2008

File:2ffl.gif

, resolution 3.33Å

Ligands:

Activity:

Ribonuclease III, with EC number 3.1.26.3

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of Dicer from Giardia intestinalis

OverviewOverview

The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end.

About this StructureAbout this Structure

2FFL is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for double-stranded RNA processing by Dicer., Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA, Science. 2006 Jan 13;311(5758):195-8. PMID:16410517

Page seeded by OCA on Thu Mar 20 16:51:49 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2ffl.gif|left|200px]]<br /><applet load="2ffl" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ffl.gif|left|200px]]
-caption="2ffl, resolution 3.33&Aring;" />
-'''Crystal Structure of Dicer from Giardia intestinalis'''<br />
+ {{Structure
+|PDB= 2ffl |SIZE=350|CAPTION= <scene name='initialview01'>2ffl</scene>, resolution 3.33&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3]
+|GENE=
+}}
+'''Crystal Structure of Dicer from Giardia intestinalis'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-FFL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFL OCA].
+FFL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFL OCA].
 ==Reference==
-Structural basis for double-stranded RNA processing by Dicer., Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA, Science. 2006 Jan 13;311(5758):195-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16410517 16410517]
+Structural basis for double-stranded RNA processing by Dicer., Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA, Science. 2006 Jan 13;311(5758):195-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16410517 16410517]
 [[Category: Giardia intestinalis]]
 [[Category: Ribonuclease III]]
@@ Line 20: / Line 29: @@
 [[Category: rna interference; rnai; paz domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:20:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:51:49 2008''