2ex5: Difference between revisions
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[[Image:2ex5.gif|left|200px]] | [[Image:2ex5.gif|left|200px]] | ||
'''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA''' | {{Structure | ||
|PDB= 2ex5 |SIZE=350|CAPTION= <scene name='initialview01'>2ex5</scene>, resolution 2.200Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= I-CeuI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3053 Chlamydomonas eugametos]) | |||
}} | |||
'''Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2EX5 is a [ | 2EX5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_eugametos Chlamydomonas eugametos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EX5 OCA]. | ||
==Reference== | ==Reference== | ||
The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold., Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL, Structure. 2006 May;14(5):869-80. PMID:[http:// | The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold., Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL, Structure. 2006 May;14(5):869-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16698548 16698548] | ||
[[Category: Chlamydomonas eugametos]] | [[Category: Chlamydomonas eugametos]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein/dna complex]] | [[Category: protein/dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:45:31 2008'' | ||
Revision as of 17:45, 20 March 2008
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| , resolution 2.200Å | |||||||
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| Ligands: | |||||||
| Gene: | I-CeuI (Chlamydomonas eugametos) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA
OverviewOverview
Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.
About this StructureAbout this Structure
2EX5 is a Single protein structure of sequence from Chlamydomonas eugametos. Full crystallographic information is available from OCA.
ReferenceReference
The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold., Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL, Structure. 2006 May;14(5):869-80. PMID:16698548
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