2efw: Difference between revisions
New page: left|200px<br /><applet load="2efw" size="350" color="white" frame="true" align="right" spinBox="true" caption="2efw, resolution 2.50Å" /> '''Crystal structure of... |
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[[Image:2efw.jpg|left|200px]] | [[Image:2efw.jpg|left|200px]] | ||
'''Crystal structure of the RTP:nRB complex from Bacillus subtilis''' | {{Structure | ||
|PDB= 2efw |SIZE=350|CAPTION= <scene name='initialview01'>2efw</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= rtp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |||
}} | |||
'''Crystal structure of the RTP:nRB complex from Bacillus subtilis''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2EFW is a [ | 2EFW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EFW OCA]. | ||
==Reference== | ==Reference== | ||
An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA., Vivian JP, Porter CJ, Wilce JA, Wilce MC, J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:[http:// | An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA., Vivian JP, Porter CJ, Wilce JA, Wilce MC, J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17521668 17521668] | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Wilce, J A.]] | [[Category: Wilce, J A.]] | ||
[[Category: Wilce, M C.J.]] | [[Category: Wilce, M C.J.]] | ||
[[Category: | [[Category: winged -helix protein]] | ||
[[Category: dna replication termination]] | [[Category: dna replication termination]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
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[[Category: replication/dna complex]] | [[Category: replication/dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:39:36 2008'' | ||
Revision as of 17:39, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Gene: | rtp (Bacillus subtilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the RTP:nRB complex from Bacillus subtilis
OverviewOverview
In Bacillus subtilis, the termination of DNA replication via polar fork arrest is effected by a specific protein:DNA complex formed between the replication terminator protein (RTP) and DNA terminator sites. We report the crystal structure of a replication terminator protein homologue (RTP.C110S) of B. subtilis in complex with the high affinity component of one of its cognate DNA termination sites, known as the TerI B-site, refined at 2.5 A resolution. The 21 bp RTP:DNA complex displays marked structural asymmetry in both the homodimeric protein and the DNA. This is in contrast to the previously reported complex formed with a symmetrical TerI B-site homologue. The induced asymmetry is consistent with the complex's solution properties as determined using NMR spectroscopy. Concomitant with this asymmetry is variation in the protein:DNA binding pattern for each of the subunits of the RTP homodimer. It is proposed that the asymmetric "wing" positions, as well as other asymmetrical features of the RTP:DNA complex, are critical for the cooperative binding that underlies the mechanism of polar fork arrest at the complete terminator site.
About this StructureAbout this Structure
2EFW is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
ReferenceReference
An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA., Vivian JP, Porter CJ, Wilce JA, Wilce MC, J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:17521668
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