2e7q: Difference between revisions
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'''Crystal structure of basic winged bean lectin in complex with b blood group trisaccharide''' | {{Structure | ||
|PDB= 2e7q |SIZE=350|CAPTION= <scene name='initialview01'>2e7q</scene>, resolution 2.75Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of basic winged bean lectin in complex with b blood group trisaccharide''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2E7Q is a [ | 2E7Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E7Q OCA]. | ||
==Reference== | ==Reference== | ||
Generation of blood group specificity: new insights from structural studies on the complexes of A- and B-reactive saccharides with basic winged bean agglutinin., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Proteins. 2007 Aug 15;68(3):762-9. PMID:[http:// | Generation of blood group specificity: new insights from structural studies on the complexes of A- and B-reactive saccharides with basic winged bean agglutinin., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Proteins. 2007 Aug 15;68(3):762-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17510954 17510954] | ||
[[Category: Psophocarpus tetragonolobus]] | [[Category: Psophocarpus tetragonolobus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: winged bean]] | [[Category: winged bean]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:36:33 2008'' | ||
Revision as of 17:36, 20 March 2008
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| , resolution 2.75Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of basic winged bean lectin in complex with b blood group trisaccharide
OverviewOverview
Basic winged bean agglutinin binds A-blood group substance with higher affinity and B-blood group substance with lesser affinity. It does not bind the O substance. The crystal structures of the lectin, complexed with A-reactive and B-reactive di and tri saccharides, have been determined. In addition, the complexes of the lectin with fucosylated A-trisaccharides and B-trisaccharides and with a variant of the A-trisaccharide have been modeled. These structures and models provide valuable insights into the structural basis of blood group specificities. All the four carbohydrate binding loops of the lectin contribute to the primary combining site while the loop of variable length contributes to the secondary binding site. In a significant advance to the current understanding, the interactions at the secondary binding site also contribute substantially, albeit in a subtle manner, to determine the blood group specificity. Compared with the interactions of the B-trisaccharide with the lectin, the third sugar residue of the A-reactive trisacharide forms an additional hydrogen bond with a lysine residue in the variable loop. In the former, the formation of such a hydrogen bond is prevented by a shift in the orientation of third sugar resulting from an internal hydrogen bond in it. The formation of this bond is also facilitated by an interaction dependent change in the rotamer conformation of the lysyl residue of the variable loop. Thus, the difference in the interactions at the secondary site is generated by coordinated movements in the ligand as well as the protein. A comparison of the crystal structure and the model of the complex involving the variant of the A-trisaccharide results in the delineation of the relative contributions of the interactions at the primary and the secondary sites in determining blood group specificity.
About this StructureAbout this Structure
2E7Q is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.
ReferenceReference
Generation of blood group specificity: new insights from structural studies on the complexes of A- and B-reactive saccharides with basic winged bean agglutinin., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Proteins. 2007 Aug 15;68(3):762-9. PMID:17510954
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