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{{STRUCTURE_3vzz|  PDB=3vzz  |  SCENE=  }}
==Crystal structure of PcrB complexed with FsPP from bacillus subtilis subap. subtilis str. 168==
===Crystal structure of PcrB complexed with FsPP from bacillus subtilis subap. subtilis str. 168===
<StructureSection load='3vzz' size='340' side='right' caption='[[3vzz]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
{{ABSTRACT_PUBMED_23322418}}
== Structural highlights ==
 
<table><tr><td colspan='2'>[[3vzz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VZZ FirstGlance]. <br>
==Function==
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FPS:S-[(2E,6E)-3,7,11-TRIMETHYLDODECA-2,6,10-TRIENYL]+TRIHYDROGEN+THIODIPHOSPHATE'>FPS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vzx|3vzx]], [[3vzy|3vzy]], [[3w00|3w00]], [[3w01|3w01]], [[3w02|3w02]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pcrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vzz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vzz RCSB], [http://www.ebi.ac.uk/pdbsum/3vzz PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PCRB_BACSU PCRB_BACSU]] Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond-formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales. To a much lesser extent, is also able to use geranyl diphosphate (GPP; C10) and geranylgeranyl diphosphate (GGPP; C20) as the prenyl donors, but not farnesyl pyrophosphate (FPP; C15). Can not use glycerol-3-phosphate (G3P) or 3-phosphoglycerate (3PG) as an acceptor.<ref>PMID:18558723</ref> <ref>PMID:21761520</ref> <ref>PMID:21214173</ref>   
[[http://www.uniprot.org/uniprot/PCRB_BACSU PCRB_BACSU]] Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond-formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales. To a much lesser extent, is also able to use geranyl diphosphate (GPP; C10) and geranylgeranyl diphosphate (GGPP; C20) as the prenyl donors, but not farnesyl pyrophosphate (FPP; C15). Can not use glycerol-3-phosphate (G3P) or 3-phosphoglycerate (3PG) as an acceptor.<ref>PMID:18558723</ref> <ref>PMID:21761520</ref> <ref>PMID:21214173</ref>   
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Well structured: As a new triose phosphate isomerase (TIM) barrel-fold prenyl transferase, PcrB catalyzes the production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate. Crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus in complex with ligands were solved, and together with site-directed mutagenesis and bioinformatics analyses, clearly reveal the catalytic mechanism of the enzyme.


==About this Structure==
Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.,Ren F, Feng X, Ko TP, Huang CH, Hu Y, Chan HC, Liu YL, Wang K, Chen CC, Pang X, He M, Li Y, Oldfield E, Guo RT Chembiochem. 2013 Jan 21;14(2):195-9. doi: 10.1002/cbic.201200748. Epub 2013 Jan , 15. PMID:23322418<ref>PMID:23322418</ref>
[[3vzz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VZZ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:023322418</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Chan, H C.]]
[[Category: Chan, H C]]
[[Category: Chen, C C.]]
[[Category: Chen, C C]]
[[Category: Feng, X.]]
[[Category: Feng, X]]
[[Category: Guo, R T.]]
[[Category: Guo, R T]]
[[Category: He, M.]]
[[Category: He, M]]
[[Category: Hu, Y.]]
[[Category: Hu, Y]]
[[Category: Huang, C H.]]
[[Category: Huang, C H]]
[[Category: Ko, T P.]]
[[Category: Ko, T P]]
[[Category: Li, Y.]]
[[Category: Li, Y]]
[[Category: Liu, Y L.]]
[[Category: Liu, Y L]]
[[Category: Oldfield, E.]]
[[Category: Oldfield, E]]
[[Category: Pang, X.]]
[[Category: Pang, X]]
[[Category: Ren, F.]]
[[Category: Ren, F]]
[[Category: Wang, K.]]
[[Category: Wang, K]]
[[Category: Biosynthesis]]
[[Category: Biosynthesis]]
[[Category: Enzyme catalysis]]
[[Category: Enzyme catalysis]]
[[Category: Prenyltransferase]]
[[Category: Prenyltransferase]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 17:47, 25 December 2014

Crystal structure of PcrB complexed with FsPP from bacillus subtilis subap. subtilis str. 168Crystal structure of PcrB complexed with FsPP from bacillus subtilis subap. subtilis str. 168

Structural highlights

3vzz is a 2 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
Gene:pcrB (Bacillus subtilis subsp. subtilis str. 168)
Resources:FirstGlance, OCA, RCSB, PDBsum

Function

[PCRB_BACSU] Prenyltransferase that catalyzes in vivo the transfer of the heptaprenyl moiety of heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P), producing heptaprenylglyceryl phosphate (HepGP). This reaction is an ether-bond-formation step in the biosynthesis of archaea-type G1P-based membrane lipids found in Bacillales. To a much lesser extent, is also able to use geranyl diphosphate (GPP; C10) and geranylgeranyl diphosphate (GGPP; C20) as the prenyl donors, but not farnesyl pyrophosphate (FPP; C15). Can not use glycerol-3-phosphate (G3P) or 3-phosphoglycerate (3PG) as an acceptor.[1] [2] [3]

Publication Abstract from PubMed

Well structured: As a new triose phosphate isomerase (TIM) barrel-fold prenyl transferase, PcrB catalyzes the production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate. Crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus in complex with ligands were solved, and together with site-directed mutagenesis and bioinformatics analyses, clearly reveal the catalytic mechanism of the enzyme.

Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.,Ren F, Feng X, Ko TP, Huang CH, Hu Y, Chan HC, Liu YL, Wang K, Chen CC, Pang X, He M, Li Y, Oldfield E, Guo RT Chembiochem. 2013 Jan 21;14(2):195-9. doi: 10.1002/cbic.201200748. Epub 2013 Jan , 15. PMID:23322418[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Guldan H, Sterner R, Babinger P. Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis. Biochemistry. 2008 Jul 15;47(28):7376-84. Epub 2008 Jun 18. PMID:18558723 doi:10.1021/bi8005779
  2. ↑ Guldan H, Matysik FM, Bocola M, Sterner R, Babinger P. Functional assignment of an enzyme that catalyzes the synthesis of an archaea-type ether lipid in bacteria. Angew Chem Int Ed Engl. 2011 Aug 22;50(35):8188-91. doi: 10.1002/anie.201101832. , Epub 2011 Jul 14. PMID:21761520 doi:10.1002/anie.201101832
  3. ↑ Doud EH, Perlstein DL, Wolpert M, Cane DE, Walker S. Two distinct mechanisms for TIM barrel prenyltransferases in bacteria. J Am Chem Soc. 2011 Feb 9;133(5):1270-3. doi: 10.1021/ja109578b. Epub 2011 Jan 7. PMID:21214173 doi:10.1021/ja109578b
  4. ↑ Ren F, Feng X, Ko TP, Huang CH, Hu Y, Chan HC, Liu YL, Wang K, Chen CC, Pang X, He M, Li Y, Oldfield E, Guo RT. Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus. Chembiochem. 2013 Jan 21;14(2):195-9. doi: 10.1002/cbic.201200748. Epub 2013 Jan , 15. PMID:23322418 doi:10.1002/cbic.201200748

3vzz, resolution 2.04Å

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