2dvg: Difference between revisions
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[[Image:2dvg.gif|left|200px]] | [[Image:2dvg.gif|left|200px]] | ||
'''Crystal structure of peanut lectin GAL-ALPHA-1,6-GLC complex''' | {{Structure | ||
|PDB= 2dvg |SIZE=350|CAPTION= <scene name='initialview01'>2dvg</scene>, resolution 2.78Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of peanut lectin GAL-ALPHA-1,6-GLC complex''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2DVG is a [ | 2DVG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arachis_hypogaea Arachis hypogaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DVG OCA]. | ||
==Reference== | ==Reference== | ||
Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin., Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:[http:// | Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin., Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17057347 17057347] | ||
[[Category: Arachis hypogaea]] | [[Category: Arachis hypogaea]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: open quaternary structure]] | [[Category: open quaternary structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:32:03 2008'' | ||
Revision as of 17:32, 20 March 2008
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| , resolution 2.78Å | |||||||
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| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of peanut lectin GAL-ALPHA-1,6-GLC complex
OverviewOverview
Crystal structures of peanut lectin complexed with Galbeta1-3Gal, methyl-T-antigen, Galbeta1-6GalNAc, Galalpha1-3Gal and Galalpha1-6Glc and that of a crystal grown in the presence of Galalpha1-3Galbeta1-4Gal have been determined using data collected at 100 K. The use of water bridges as a strategy for generating carbohydrate specificity was previously deduced from the complexes of the lectin with lactose (Galbeta1-4Glc) and T-antigen (Galbeta1-3GalNAc). This has been confirmed by the analysis of the complexes with Galbeta1-3Gal and methyl-T-antigen (Galbeta1-3GalNAc-alpha-OMe). A detailed analysis of lectin-sugar interactions in the complexes shows that they are more extensive when the beta-anomer is involved in the linkage. As expected, the second sugar residue is ill-defined when the linkage is 1-->6. There are more than two dozen water molecules which occur in the hydration shells of all structures determined at resolutions better than 2.5 A. Most of them are involved in stabilizing the structure, particularly loops. Water molecules involved in lectin-sugar interactions are also substantially conserved. The lectin molecule is fairly rigid and does not appear to be affected by changes in temperature.
About this StructureAbout this Structure
2DVG is a Single protein structure of sequence from Arachis hypogaea. Full crystallographic information is available from OCA.
ReferenceReference
Structural studies on peanut lectin complexed with disaccharides involving different linkages: further insights into the structure and interactions of the lectin., Natchiar SK, Srinivas O, Mitra N, Surolia A, Jayaraman N, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2006 Nov;62(Pt 11):1413-21. Epub 2006, Oct 18. PMID:17057347
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