2dsw: Difference between revisions

Revision as of 17:31, 20 March 2008

, resolution 2.80Å

Ligands:

Coordinates:

Binding of chitin-like polysaccharides to protective signalling factor: crystal structure of the complex of signalling protein from sheep (SPS-40) with a pentasaccharide at 2.8 A resolution

OverviewOverview

Crystal structures of four complexes of sheep secretory glycoprotein (SPS-40) with N-acetylglucosamine oligosaccharides (GlcNAc(n), (n=3-6)) have been determined at moderate resolutions. The binding studies of SPS-40 have been carried out using fluorescence spectroscopy and Surface Plasmon Resonance (SPR). Structure determinations of four complexes have shown a novel binding pattern of GlcNAc(n) molecules to SPS-40. The results indicate that the most preferred recognition region in the carbohydrate binding groove in SPS-40 is at subsites -4 to -2 among which subsite -2 provides the maximum interactions with carbohydrate residues. These structures have also shown that the interactions of GlcNAc3 and GlcNAc4 do not perturb the protein structure and those of GlcNAc5 induce partial conformational changes while in the case of GlcNAc6 the partially closed binding groove opened up completely. As in other SPX-40 structures, SPS-40 structure contains three overlapping flexible surface segments, His188-His197, Phe202-Arg212 and Phe244-Pro260 with several charged residues protruding outwardly. It creates a cluster of positive charges with a flexible base thus indicating a good scope of promoting the intermolecular interactions. This protein is glycosylated at Asn39 and may recognize other receptors having sugar binding sites. It appears that SPS-40 may involve both carbohydrate and protein bindings. The systematic carbohydrate-binding studies and the detailed structural results of four protein-carbohydrate complexes provide an excellent insight into the mechanism of carbohydrate binding. These are the first studies of this kind on secretory glycoproteins and their interactions with carbohydrates.

About this StructureAbout this Structure

2DSW is a Single protein structure of sequence from Ovis aries. This structure supersedes the now removed PDB entries 2B2P and 2AOK. Full crystallographic information is available from OCA.

ReferenceReference

Carbohydrate binding properties and carbohydrate induced conformational switch in sheep secretory glycoprotein (SPS-40): crystal structures of four complexes of SPS-40 with chitin-like oligosaccharides., Srivastava DB, Ethayathulla AS, Kumar J, Somvanshi RK, Sharma S, Dey S, Singh TP, J Struct Biol. 2007 Jun;158(3):255-66. Epub 2006 Nov 17. PMID:17188513

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OCA

@@ Line 1: / Line 1: @@
-[[Image:2dsw.gif|left|200px]]<br /><applet load="2dsw" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2dsw.gif|left|200px]]
-caption="2dsw, resolution 2.80&Aring;" />
-'''Binding of chitin-like polysaccharides to protective signalling factor: crystal structure of the complex of signalling protein from sheep (SPS-40) with a pentasaccharide at 2.8 A resolution'''<br />
+ {{Structure
+|PDB= 2dsw |SIZE=350|CAPTION= <scene name='initialview01'>2dsw</scene>, resolution 2.80&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Binding of chitin-like polysaccharides to protective signalling factor: crystal structure of the complex of signalling protein from sheep (SPS-40) with a pentasaccharide at 2.8 A resolution'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DSW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entries 2B2P and 2AOK. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSW OCA].
+DSW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. This structure supersedes the now removed PDB entries 2B2P and 2AOK. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DSW OCA].
 ==Reference==
-Carbohydrate binding properties and carbohydrate induced conformational switch in sheep secretory glycoprotein (SPS-40): crystal structures of four complexes of SPS-40 with chitin-like oligosaccharides., Srivastava DB, Ethayathulla AS, Kumar J, Somvanshi RK, Sharma S, Dey S, Singh TP, J Struct Biol. 2007 Jun;158(3):255-66. Epub 2006 Nov 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17188513 17188513]
+Carbohydrate binding properties and carbohydrate induced conformational switch in sheep secretory glycoprotein (SPS-40): crystal structures of four complexes of SPS-40 with chitin-like oligosaccharides., Srivastava DB, Ethayathulla AS, Kumar J, Somvanshi RK, Sharma S, Dey S, Singh TP, J Struct Biol. 2007 Jun;158(3):255-66. Epub 2006 Nov 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17188513 17188513]
 [[Category: Ovis aries]]
 [[Category: Single protein]]
@@ Line 26: / Line 35: @@
 [[Category: sps-40]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:02:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:31:09 2008''