2dgc: Difference between revisions

Revision as of 17:26, 20 March 2008

, resolution 2.200Å

Coordinates:

GCN4 BASIC DOMAIN, LEUCINE ZIPPER COMPLEXED WITH ATF/CREB SITE DNA

OverviewOverview

The X-ray structure of the GCN4-bZIP protein bound to DNA containing the ATF/CREB recognition sequence has been refined at 2.2 A. The water-mediated interactions between the basic domain and DNA are revealed, and combined with a more accurate description of the direct contacts, further clarify how binding specificity is achieved. Water molecules extend the interactions of both invariant basic domain residues, asparagine 235 and arginine 243, beyond their direct base contacts. The slight bending of the basic domain alpha-helix around the DNA facilitates the linking of arginine 241, 243 and 245 to main-chain carbonyl oxygen atoms via water molecules, apparently stabilizing interactions with the DNA.

About this StructureAbout this Structure

2DGC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a bZIP/DNA complex at 2.2 A: determinants of DNA specific recognition., Keller W, Konig P, Richmond TJ, J Mol Biol. 1995 Dec 8;254(4):657-67. PMID:7500340

Page seeded by OCA on Thu Mar 20 16:26:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2dgc.gif|left|200px]]<br /><applet load="2dgc" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2dgc.gif|left|200px]]
-caption="2dgc, resolution 2.200&Aring;" />
-'''GCN4 BASIC DOMAIN, LEUCINE ZIPPER COMPLEXED WITH ATF/CREB SITE DNA'''<br />
+ {{Structure
+|PDB= 2dgc |SIZE=350|CAPTION= <scene name='initialview01'>2dgc</scene>, resolution 2.200&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''GCN4 BASIC DOMAIN, LEUCINE ZIPPER COMPLEXED WITH ATF/CREB SITE DNA'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DGC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DGC OCA].
+DGC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DGC OCA].
 ==Reference==
-Crystal structure of a bZIP/DNA complex at 2.2 A: determinants of DNA specific recognition., Keller W, Konig P, Richmond TJ, J Mol Biol. 1995 Dec 8;254(4):657-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7500340 7500340]
+Crystal structure of a bZIP/DNA complex at 2.2 A: determinants of DNA specific recognition., Keller W, Konig P, Richmond TJ, J Mol Biol. 1995 Dec 8;254(4):657-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7500340 7500340]
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
@@ Line 21: / Line 30: @@
 [[Category: leucine zipper]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:58:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:26:55 2008''