2ddk: Difference between revisions

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Revision as of 17:26, 20 March 2008

File:2ddk.gif

, resolution 2.70Å

Gene:

IMPA2, IMP.18P (Homo sapiens)

Activity:

Inositol-phosphate phosphatase, with EC number 3.1.3.25

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of human myo-inositol monophosphatase 2 (IMPA2) (orthorhombic form)

OverviewOverview

The human IMPA2 gene, which encodes myo-inositol monophosphatase 2 (IMPA2), is mapped onto 18p11.2, a susceptibility region for bipolar disorder. This chromosomal region has also been proposed to include a susceptibility locus for schizophrenia and febrile seizures. Here we report the crystal structures of human IMPA2 and its complex with calcium and phosphate ions. Human IMPA2 comprises an alpha-beta protein with a five-layered sandwich of alpha-helices and beta-sheets (alpha-beta-alpha-beta-alpha). The crystal structure and analytical ultracentrifugation results indicated that IMPA2 exists as a dimer in solution. The overall structure of IMPA2 is similar to that of IMPA1, except for the loop regions. In IMPA1, the loop region (31-43) is located at the entrance of the active site cavity. In the corresponding region (42-54) of IMPA2, the residues are disordered and partially form an alpha-helix. The structural difference in the opening of the active site cavity suggests that the substrate specificity differs between IMPA1 and IMPA2. The widely opened cavity of IMPA2 implies that the physiological substrate may be a larger compound than inositol monophosphate. The structure of IMPA2 complexed with Ca2+ revealed two metals and one phosphate binding sites, which were the same sites as in IMPA1 complexed with Mn2+ and phosphate, suggesting that the mechanism of the enzymatic reaction is similar to that of IMPA1. The crystal structures of human IMPA2 are useful for understanding the effect of nonsynonymous polymorphism reported in IMPA2, and will contribute to further functional analyses of IMPA2 that potentially predisposes to the vulnerabilities of bipolar disorder, schizophrenia, and febrile seizures.

About this StructureAbout this Structure

2DDK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures., Arai R, Ito K, Ohnishi T, Ohba H, Akasaka R, Bessho Y, Hanawa-Suetsugu K, Yoshikawa T, Shirouzu M, Yokoyama S, Proteins. 2007 May 15;67(3):732-42. PMID:17340635

Page seeded by OCA on Thu Mar 20 16:25:58 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2ddk.gif|left|200px]]<br /><applet load="2ddk" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ddk.gif|left|200px]]
-caption="2ddk, resolution 2.70&Aring;" />
-'''Crystal structure of human myo-inositol monophosphatase 2 (IMPA2) (orthorhombic form)'''<br />
+ {{Structure
+|PDB= 2ddk |SIZE=350|CAPTION= <scene name='initialview01'>2ddk</scene>, resolution 2.70&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/Inositol-phosphate_phosphatase Inositol-phosphate phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.25 3.1.3.25]
+|GENE= IMPA2, IMP.18P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''Crystal structure of human myo-inositol monophosphatase 2 (IMPA2) (orthorhombic form)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DDK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Inositol-phosphate_phosphatase Inositol-phosphate phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.25 3.1.3.25] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DDK OCA].
+DDK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DDK OCA].
 ==Reference==
-Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures., Arai R, Ito K, Ohnishi T, Ohba H, Akasaka R, Bessho Y, Hanawa-Suetsugu K, Yoshikawa T, Shirouzu M, Yokoyama S, Proteins. 2007 May 15;67(3):732-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17340635 17340635]
+Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures., Arai R, Ito K, Ohnishi T, Ohba H, Akasaka R, Bessho Y, Hanawa-Suetsugu K, Yoshikawa T, Shirouzu M, Yokoyama S, Proteins. 2007 May 15;67(3):732-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17340635 17340635]
 [[Category: Homo sapiens]]
 [[Category: Inositol-phosphate phosphatase]]
@@ Line 25: / Line 34: @@
 [[Category: Yoshikawa, T.]]
 [[Category: bipolar disorder]]
-[[Category: febrile seizures]]
+[[Category: febrile seizure]]
 [[Category: myo-inositol monophosphatase (impa)]]
-[[Category: national project on protein structural and functional analyses]]
+[[Category: national project on protein structural and functional analyse]]
 [[Category: nppsfa]]
 [[Category: riken structural genomics/proteomics initiative]]
 [[Category: rsgi]]
 [[Category: schizophrenia]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:57:44 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:25:58 2008''