4k3b: Difference between revisions
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==The crystal structure of BamA from Neisseria gonorrhoeae== | |||
=== | <StructureSection load='4k3b' size='340' side='right' caption='[[4k3b]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4k3b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae_fa_1090 Neisseria gonorrhoeae fa 1090]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K3B FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4k3c|4k3c]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bamA, NGO1801 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=242231 Neisseria gonorrhoeae FA 1090])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k3b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k3b RCSB], [http://www.ebi.ac.uk/pdbsum/4k3b PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/Q5F5W8_NEIG1 Q5F5W8_NEIG1]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane (By similarity).[HAMAP-Rule:MF_01430] | [[http://www.uniprot.org/uniprot/Q5F5W8_NEIG1 Q5F5W8_NEIG1]] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane (By similarity).[HAMAP-Rule:MF_01430] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
beta-barrel membrane proteins are essential for nutrient import, signalling, motility and survival. In Gram-negative bacteria, the beta-barrel assembly machinery (BAM) complex is responsible for the biogenesis of beta-barrel membrane proteins, with homologous complexes found in mitochondria and chloroplasts. Here we describe the structure of BamA, the central and essential component of the BAM complex, from two species of bacteria: Neisseria gonorrhoeae and Haemophilus ducreyi. BamA consists of a large periplasmic domain attached to a 16-strand transmembrane beta-barrel domain. Three structural features shed light on the mechanism by which BamA catalyses beta-barrel assembly. First, the interior cavity is accessible in one BamA structure and conformationally closed in the other. Second, an exterior rim of the beta-barrel has a distinctly narrowed hydrophobic surface, locally destabilizing the outer membrane. And third, the beta-barrel can undergo lateral opening, suggesting a route from the interior cavity in BamA into the outer membrane. | |||
Structural insight into the biogenesis of beta-barrel membrane proteins.,Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK Nature. 2013 Sep 1. doi: 10.1038/nature12521. PMID:23995689<ref>PMID:23995689</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Neisseria gonorrhoeae fa 1090]] | [[Category: Neisseria gonorrhoeae fa 1090]] | ||
[[Category: Buchanan, S K | [[Category: Buchanan, S K]] | ||
[[Category: Chang, H | [[Category: Chang, H]] | ||
[[Category: Easley, N | [[Category: Easley, N]] | ||
[[Category: Lukacik, P | [[Category: Lukacik, P]] | ||
[[Category: Noinaj, N | [[Category: Noinaj, N]] | ||
[[Category: Beta-barrel membrane protein]] | [[Category: Beta-barrel membrane protein]] | ||
[[Category: Insertase]] | [[Category: Insertase]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
Revision as of 15:19, 25 December 2014
The crystal structure of BamA from Neisseria gonorrhoeaeThe crystal structure of BamA from Neisseria gonorrhoeae
Structural highlights
Function[Q5F5W8_NEIG1] Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane (By similarity).[HAMAP-Rule:MF_01430] Publication Abstract from PubMedbeta-barrel membrane proteins are essential for nutrient import, signalling, motility and survival. In Gram-negative bacteria, the beta-barrel assembly machinery (BAM) complex is responsible for the biogenesis of beta-barrel membrane proteins, with homologous complexes found in mitochondria and chloroplasts. Here we describe the structure of BamA, the central and essential component of the BAM complex, from two species of bacteria: Neisseria gonorrhoeae and Haemophilus ducreyi. BamA consists of a large periplasmic domain attached to a 16-strand transmembrane beta-barrel domain. Three structural features shed light on the mechanism by which BamA catalyses beta-barrel assembly. First, the interior cavity is accessible in one BamA structure and conformationally closed in the other. Second, an exterior rim of the beta-barrel has a distinctly narrowed hydrophobic surface, locally destabilizing the outer membrane. And third, the beta-barrel can undergo lateral opening, suggesting a route from the interior cavity in BamA into the outer membrane. Structural insight into the biogenesis of beta-barrel membrane proteins.,Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK Nature. 2013 Sep 1. doi: 10.1038/nature12521. PMID:23995689[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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