4h2b: Difference between revisions

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-[[Image:4h2b.png|left|200px]]
+==Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with Baicalin==
+<StructureSection load='4h2b' size='340' side='right' caption='[[4h2b]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4h2b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H2B FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0XE:5,6-DIHYDROXY-4-OXO-2-PHENYL-4H-CHROMEN-7-YL+BETA-D-GLUCOPYRANOSIDURONIC+ACID'>0XE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h1y|4h1y]], [[4h2f|4h2f]], [[4h2i|4h2i]], [[4h2g|4h2g]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NT5, NT5E, NTE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h2b RCSB], [http://www.ebi.ac.uk/pdbsum/4h2b PDBsum]</span></td></tr>
+</table>
+== Disease ==
+[[http://www.uniprot.org/uniprot/5NTD_HUMAN 5NTD_HUMAN]] Hereditary arterial and articular multiple calcification syndrome. The disease is caused by mutations affecting the gene represented in this entry.
+== Function ==
+[[http://www.uniprot.org/uniprot/5NTD_HUMAN 5NTD_HUMAN]] Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.<ref>PMID:21933152</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In vertebrates ecto-5'-nucleotidase (e5NT) catalyzes the hydrolysis of extracellular AMP to adenosine and represents the major control point for extracellular adenosine levels. Due to its pivotal role for activation of P1 adenosine receptors, e5NT has emerged as an appealing drug target for treatment of inflammation, chronic pain, hypoxia, and cancer. Crystal structures of the dimeric human e5NT reveal an extensive 114 degrees conformational switch between the open and closed forms of the enzyme. The dimerization interface is formed by the C-terminal domains and exhibits interchain motions of up to 13 degrees . Complex structures with adenosine and AMPCP indicate that structural control of the domain movement determines the selectivity for monophosphate nucleotides. Binding modes of nucleotide-derived and flavonoid-based compounds complexed to the C-terminal domain in the open form reveal an additional binding pocket of approximately 210 A(3) that might be explored to design more potent inhibitors.
-{{STRUCTURE_4h2b|  PDB=4h2b  |  SCENE=  }}
+Crystal Structure of the Human Ecto-5'-Nucleotidase (CD73): Insights into the Regulation of Purinergic Signaling.,Knapp K, Zebisch M, Pippel J, El-Tayeb A, Muller CE, Strater N Structure. 2012 Nov 6. pii: S0969-2126(12)00375-9. doi:, 10.1016/j.str.2012.10.001. PMID:23142347<ref>PMID:23142347</ref>
-===Human ecto-5'-nucleotidase (CD73): crystal form II (open) in complex with Baicalin===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_23142347}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[4h2b]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2B OCA].
+</StructureSection>
 [[Category: 5'-nucleotidase]]
 [[Category: Homo sapiens]]
-[[Category: Knapp, K.]]
+[[Category: Knapp, K]]
-[[Category: Pippel, J.]]
+[[Category: Pippel, J]]
-[[Category: Straeter, N.]]
+[[Category: Straeter, N]]
-[[Category: Zebisch, M.]]
+[[Category: Zebisch, M]]
 [[Category: Dimer]]
 [[Category: Extracellular]]
 [[Category: Hydrolase phosphatase nucleotidase]]
 [[Category: Hydrolase-hydrolase inhibitor complex]]