4hz3: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hz3 RCSB], [http://www.ebi.ac.uk/pdbsum/4hz3 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hz3 RCSB], [http://www.ebi.ac.uk/pdbsum/4hz3 PDBsum]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel. | |||
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== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Revision as of 12:26, 25 December 2014
MthK pore crystallized in presence of TBSbMthK pore crystallized in presence of TBSb
Structural highlights
Function[MTHK_METTH] Calcium-gated potassium channel. Publication Abstract from PubMedUnderstanding how ion channels open and close their pores is crucial for comprehending their physiological roles. We used intracellular quaternary ammonium blockers, electrophysiology and X-ray crystallography to locate the voltage-dependent gate in MthK potassium channels from Methanobacterium thermoautotrophicum. Blockers bind in an aqueous cavity between two putative gates: an intracellular gate and the selectivity filter. Thus, these blockers directly probe gate location-an intracellular gate will prevent binding when closed, whereas a selectivity filter gate will always allow binding. Kinetic analysis of tetrabutylammonium block of single MthK channels combined with X-ray crystallographic analysis of the pore with tetrabutyl antimony unequivocally determined that the voltage-dependent gate, like the C-type inactivation gate in eukaryotic channels, is located at the selectivity filter. State-dependent binding kinetics suggest that MthK inactivation leads to conformational changes within the cavity and intracellular pore entrance. The voltage-dependent gate in MthK potassium channels is located at the selectivity filter.,Posson DJ, McCoy JG, Nimigean CM Nat Struct Mol Biol. 2013 Feb;20(2):159-66. doi: 10.1038/nsmb.2473. Epub 2012 Dec, 23. PMID:23262489[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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