2cko: Difference between revisions
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[[Image:2cko.gif|left|200px]] | [[Image:2cko.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF HUMAN CHOLINE KINASE ALPHA 2''' | {{Structure | ||
|PDB= 2cko |SIZE=350|CAPTION= <scene name='initialview01'>2cko</scene>, resolution 2.15Å | |||
|SITE= <scene name='pdbsite=AC1:Dtt+Binding+Site+For+Chain+B'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Choline_kinase Choline kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.32 2.7.1.32] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF HUMAN CHOLINE KINASE ALPHA 2''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CKO is a [ | 2CKO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CKO OCA]. | ||
==Reference== | ==Reference== | ||
Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine., Malito E, Sekulic N, Too WC, Konrad M, Lavie A, J Mol Biol. 2006 Nov 24;364(2):136-51. Epub 2006 Sep 3. PMID:[http:// | Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine., Malito E, Sekulic N, Too WC, Konrad M, Lavie A, J Mol Biol. 2006 Nov 24;364(2):136-51. Epub 2006 Sep 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17007874 17007874] | ||
[[Category: Choline kinase]] | [[Category: Choline kinase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:16:36 2008'' | ||
Revision as of 17:16, 20 March 2008
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| , resolution 2.15Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Choline kinase, with EC number 2.7.1.32 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF HUMAN CHOLINE KINASE ALPHA 2
OverviewOverview
Choline kinase, responsible for the phosphorylation of choline to phosphocholine as the first step of the CDP-choline pathway for the biosynthesis of phosphatidylcholine, has been recognized as a new target for anticancer therapy. Crystal structures of human choline kinase in its apo, ADP and phosphocholine-bound complexes, respectively, reveal the molecular details of the substrate binding sites. ATP binds in a cavity where residues from both the N and C-terminal lobes contribute to form a cleft, while the choline-binding site constitutes a deep hydrophobic groove in the C-terminal domain with a rim composed of negatively charged residues. Upon binding of choline, the enzyme undergoes conformational changes independently affecting the N-terminal domain and the ATP-binding loop. From this structural analysis and comparison with other kinases, and from mutagenesis data on the homologous Caenorhabditis elegans choline kinase, a model of the ternary ADP.phosphocholine complex was built that reveals the molecular basis for the phosphoryl transfer activity of this enzyme.
DiseaseDisease
Known diseases associated with this structure: Breast and colorectal cancer, susceptibility to OMIM:[604373], Breast cancer, susceptibility to OMIM:[604373], Li-Fraumeni syndrome OMIM:[604373], Osteosarcoma, somatic OMIM:[604373], Prostate cancer, familial OMIM:[604373]
About this StructureAbout this Structure
2CKO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine., Malito E, Sekulic N, Too WC, Konrad M, Lavie A, J Mol Biol. 2006 Nov 24;364(2):136-51. Epub 2006 Sep 3. PMID:17007874
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