2cci: Difference between revisions
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[[Image:2cci.gif|left|200px]] | [[Image:2cci.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF PHOSPHO-CDK2 CYCLIN A IN COMPLEX WITH A PEPTIDE CONTAINING BOTH THE SUBSTRATE AND RECRUITMENT SITES OF CDC6''' | {{Structure | ||
|PDB= 2cci |SIZE=350|CAPTION= <scene name='initialview01'>2cci</scene>, resolution 2.70Å | |||
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+C'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_2.7.11.1 Transferred entry: 2.7.11.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.37 2.7.1.37] | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF PHOSPHO-CDK2 CYCLIN A IN COMPLEX WITH A PEPTIDE CONTAINING BOTH THE SUBSTRATE AND RECRUITMENT SITES OF CDC6''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2CCI is a [ | 2CCI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CCI OCA]. | ||
==Reference== | ==Reference== | ||
The role of the phospho-CDK2/cyclin A recruitment site in substrate recognition., Cheng KY, Noble ME, Skamnaki V, Brown NR, Lowe ED, Kontogiannis L, Shen K, Cole PA, Siligardi G, Johnson LN, J Biol Chem. 2006 Aug 11;281(32):23167-79. Epub 2006 May 17. PMID:[http:// | The role of the phospho-CDK2/cyclin A recruitment site in substrate recognition., Cheng KY, Noble ME, Skamnaki V, Brown NR, Lowe ED, Kontogiannis L, Shen K, Cole PA, Siligardi G, Johnson LN, J Biol Chem. 2006 Aug 11;281(32):23167-79. Epub 2006 May 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16707497 16707497] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: nucleotide-binding]] | [[Category: nucleotide-binding]] | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
[[Category: protein | [[Category: protein kinase]] | ||
[[Category: recruitment]] | [[Category: recruitment]] | ||
[[Category: serine/threonine-protein kinase]] | [[Category: serine/threonine-protein kinase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:13:44 2008'' | ||
Revision as of 17:13, 20 March 2008
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| , resolution 2.70Å | |||||||
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| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | Transferred entry: 2.7.11.1, with EC number 2.7.1.37 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF PHOSPHO-CDK2 CYCLIN A IN COMPLEX WITH A PEPTIDE CONTAINING BOTH THE SUBSTRATE AND RECRUITMENT SITES OF CDC6
OverviewOverview
Phospho-CDK2/cyclin A, a kinase that is active in cell cycle S phase, contains an RXL substrate recognition site that is over 40 A from the catalytic site. The role of this recruitment site, which enhances substrate affinity and catalytic efficiency, has been investigated using peptides derived from the natural substrates, namely CDC6 and p107, and a bispeptide inhibitor in which the gamma-phosphate of ATP is covalently attached by a linker to the CDC6 substrate peptide. X-ray studies with a 30-residue CDC6 peptide in complex with pCDK2/cyclin A showed binding of a dodecamer peptide at the recruitment site and a heptapeptide at the catalytic site, but no density for the linking 11 residues. Kinetic studies established that the CDC6 peptide had an 18-fold lower Km compared with heptapeptide substrate and that this effect required the recruitment peptide to be covalently linked to the substrate peptide. X-ray studies with the CDC6 bispeptide showed binding of the dodecamer at the recruitment site and the modified ATP in two alternative conformations at the catalytic site. The CDC6 bispeptide was a potent inhibitor competitive with both ATP and peptide substrate of pCDK2/cyclin A activity against a heptapeptide substrate (Ki = 0.83 nm) but less effective against RXL-containing substrates. We discuss how localization at the recruitment site (KD 0.4 microm) leads to increased catalytic efficiency and the design of a potent inhibitor. The notion of a flexible linker between the sites, which must have more than a minimal number of residues, provides an explanation for recognition and discrimination against different substrates.
About this StructureAbout this Structure
2CCI is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
The role of the phospho-CDK2/cyclin A recruitment site in substrate recognition., Cheng KY, Noble ME, Skamnaki V, Brown NR, Lowe ED, Kontogiannis L, Shen K, Cole PA, Siligardi G, Johnson LN, J Biol Chem. 2006 Aug 11;281(32):23167-79. Epub 2006 May 17. PMID:16707497
Page seeded by OCA on Thu Mar 20 16:13:44 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Homo sapiens
- Protein complex
- Transferred entry: 2 7.11 1
- Brown, N R.
- Cheng, K Y.
- Cole, P A.
- Johnson, L N.
- Kontogiannis, L.
- Lowe, E D.
- Noble, M E.M.
- Shen, K.
- Siligardi, G.
- Skamnaki, V.
- ATP
- MG
- Atp-binding
- Cell cycle
- Cell division
- Complex
- Cyclin
- Dna replication
- Kinase
- Mitosis
- Nuclear protein
- Nucleotide-binding
- Phosphorylation
- Protein kinase
- Recruitment
- Serine/threonine-protein kinase
- Substrate recognition
- Transferase