2caz: Difference between revisions

Revision as of 17:13, 20 March 2008

, resolution 3.60Å

Coordinates:

ESCRT-I CORE

OverviewOverview

ESCRT complexes form the main machinery driving protein sorting from endosomes to lysosomes. Currently, the picture regarding assembly of ESCRTs on endosomes is incomplete. The structure of the conserved heterotrimeric ESCRT-I core presented here shows a fan-like arrangement of three helical hairpins, each corresponding to a different subunit. Vps23/Tsg101 is the central hairpin sandwiched between the other subunits, explaining the critical role of its "steadiness box" in the stability of ESCRT-I. We show that yeast ESCRT-I links directly to ESCRT-II, through a tight interaction of Vps28 (ESCRT-I) with the yeast-specific zinc-finger insertion within the GLUE domain of Vps36 (ESCRT-II). The crystal structure of the GLUE domain missing this insertion reveals it is a split PH domain, with a noncanonical lipid binding pocket that binds PtdIns3P. The simultaneous and reinforcing interactions of ESCRT-II GLUE domain with membranes, ESCRT-I, and ubiquitin are critical for ubiquitinated cargo progression from early to late endosomes.

About this StructureAbout this Structure

2CAZ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes., Teo H, Gill DJ, Sun J, Perisic O, Veprintsev DB, Vallis Y, Emr SD, Williams RL, Cell. 2006 Apr 7;125(1):99-111. PMID:16615893

Page seeded by OCA on Thu Mar 20 16:13:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2caz.gif|left|200px]]<br /><applet load="2caz" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2caz.gif|left|200px]]
-caption="2caz, resolution 3.60&Aring;" />
-'''ESCRT-I CORE'''<br />
+ {{Structure
+|PDB= 2caz |SIZE=350|CAPTION= <scene name='initialview01'>2caz</scene>, resolution 3.60&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''ESCRT-I CORE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-CAZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CAZ OCA].
+CAZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CAZ OCA].
 ==Reference==
-ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes., Teo H, Gill DJ, Sun J, Perisic O, Veprintsev DB, Vallis Y, Emr SD, Williams RL, Cell. 2006 Apr 7;125(1):99-111. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16615893 16615893]
+ESCRT-I core and ESCRT-II GLUE domain structures reveal role for GLUE in linking to ESCRT-I and membranes., Teo H, Gill DJ, Sun J, Perisic O, Veprintsev DB, Vallis Y, Emr SD, Williams RL, Cell. 2006 Apr 7;125(1):99-111. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16615893 16615893]
 [[Category: Protein complex]]
 [[Category: Saccharomyces cerevisiae]]
@@ Line 25: / Line 34: @@
 [[Category: escrt]]
 [[Category: lysosome]]
-[[Category: multivesicular bodies]]
+[[Category: multivesicular body]]
 [[Category: mvb]]
 [[Category: ph domain]]
@@ Line 40: / Line 49: @@
 [[Category: vps37]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:46:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:13:07 2008''