2c4b: Difference between revisions

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Revision as of 17:10, 20 March 2008

File:2c4b.gif

, resolution 1.30Å

Sites:

Ligands:

, , , , , and

Activity:

Ribonuclease T(1), with EC number 3.1.27.3

Coordinates:

save as pdb, mmCIF, xml

INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A

OverviewOverview

We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.

About this StructureAbout this Structure

2C4B is a Single protein structure of sequence from Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium. Full crystallographic information is available from OCA.

ReferenceReference

Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652

Page seeded by OCA on Thu Mar 20 16:10:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2c4b.gif|left|200px]]<br /><applet load="2c4b" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2c4b.gif|left|200px]]
-caption="2c4b, resolution 1.30&Aring;" />
-'''INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A'''<br />
+ {{Structure
+|PDB= 2c4b |SIZE=350|CAPTION= <scene name='initialview01'>2c4b</scene>, resolution 1.30&Aring;
+|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]
+|GENE=
+}}
+'''INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-C4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens,_momordica_cochinchinensis,_ecballium_elaterium Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=2PE:'>2PE</scene>, <scene name='pdbligand=EDO:'>EDO</scene>, <scene name='pdbligand=MES:'>MES</scene>, <scene name='pdbligand=UNX:'>UNX</scene>, <scene name='pdbligand=GOL:'>GOL</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA].
+C4B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens,_momordica_cochinchinensis,_ecballium_elaterium Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA].
 ==Reference==
-Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16337652 16337652]
+Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16337652 16337652]
 [[Category: Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]]
 [[Category: Ribonuclease T(1)]]
@@ Line 36: / Line 45: @@
 [[Category: squash inhibitor]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:44:43 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:10:36 2008''