2bra: Difference between revisions
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[[Image:2bra.gif|left|200px]] | [[Image:2bra.gif|left|200px]] | ||
'''STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL''' | {{Structure | ||
|PDB= 2bra |SIZE=350|CAPTION= <scene name='initialview01'>2bra</scene>, resolution 2.00Å | |||
|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+B'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2BRA is a [ | 2BRA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BRA OCA]. | ||
==Reference== | ==Reference== | ||
Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:[http:// | Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16275926 16275926] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:05:39 2008'' | ||
Revision as of 17:05, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL
OverviewOverview
During development, neurons are guided to their targets by short- and long-range attractive and repulsive cues. MICAL, a large multidomain protein, is required for the combined action of semaphorins and plexins in axon guidance. Here, we present the structure of the N-terminal region of MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The structure shows that MICAL(fd) is an FAD-containing module structurally similar to aromatic hydroxylases and amine oxidases. In addition, we present biochemical data that show that MICAL(fd) is a flavoenzyme that in the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) = 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling properties. We propose that the H(2)O(2) produced by this reaction may be one of the signaling molecules involved in axon guidance by MICAL.
About this StructureAbout this Structure
2BRA is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:16275926
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