2bjw: Difference between revisions

Revision as of 17:02, 20 March 2008

, resolution 1.75Å

Coordinates:

PSPF AAA DOMAIN

OverviewOverview

Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.

About this StructureAbout this Structure

2BJW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859

Page seeded by OCA on Thu Mar 20 16:02:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2bjw.gif|left|200px]]<br /><applet load="2bjw" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bjw.gif|left|200px]]
-caption="2bjw, resolution 1.75&Aring;" />
-'''PSPF AAA DOMAIN'''<br />
+ {{Structure
+|PDB= 2bjw |SIZE=350|CAPTION= <scene name='initialview01'>2bjw</scene>, resolution 1.75&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''PSPF AAA DOMAIN'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-BJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJW OCA].
+BJW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJW OCA].
 ==Reference==
-Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15790859 15790859]
+Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15790859 15790859]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 33: / Line 42: @@
 [[Category: transcription regulation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:34 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:02:52 2008''