2bj0: Difference between revisions
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[[Image:2bj0.gif|left|200px]] | [[Image:2bj0.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF ACHBP FROM BULINUS TRUNCATUS REVALS THE CONSERVED STRUCTURAL SCAFFOLD AND SITES OF VARIATION IN NICOTINIC ACETYLCHOLINE RECEPTORS''' | {{Structure | ||
|PDB= 2bj0 |SIZE=350|CAPTION= <scene name='initialview01'>2bj0</scene>, resolution 2.00Å | |||
|SITE= <scene name='pdbsite=AC1:Cxs+Binding+Site+For+Chain+D'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC ACID'>CXS</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF ACHBP FROM BULINUS TRUNCATUS REVALS THE CONSERVED STRUCTURAL SCAFFOLD AND SITES OF VARIATION IN NICOTINIC ACETYLCHOLINE RECEPTORS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2BJ0 is a [ | 2BJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bulinus_truncatus Bulinus truncatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJ0 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of acetylcholine-binding protein from Bulinus truncatus reveals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors., Celie PH, Klaassen RV, van Rossum-Fikkert SE, van Elk R, van Nierop P, Smit AB, Sixma TK, J Biol Chem. 2005 Jul 15;280(28):26457-66. Epub 2005 May 16. PMID:[http:// | Crystal structure of acetylcholine-binding protein from Bulinus truncatus reveals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors., Celie PH, Klaassen RV, van Rossum-Fikkert SE, van Elk R, van Nierop P, Smit AB, Sixma TK, J Biol Chem. 2005 Jul 15;280(28):26457-66. Epub 2005 May 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15899893 15899893] | ||
[[Category: Bulinus truncatus]] | [[Category: Bulinus truncatus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: signal]] | [[Category: signal]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:02:41 2008'' | ||
Revision as of 17:02, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF ACHBP FROM BULINUS TRUNCATUS REVALS THE CONSERVED STRUCTURAL SCAFFOLD AND SITES OF VARIATION IN NICOTINIC ACETYLCHOLINE RECEPTORS
OverviewOverview
The crystal structure of acetylcholine-binding protein (AChBP) from the mollusk Lymnaea stagnalis is the established model for the ligand binding domains of the ligand-gated ion channel family, which includes nicotinic acetylcholine, 5-hydroxytryptamine (5-HT3), gamma-aminobutyric acid (GABA), types A and C, and glycine receptors. Here we present the crystal structure of a remote homolog, AChBP from Bulinus truncatus, which reveals both the conserved structural scaffold and the sites of variation in this receptor family. These include rigid body movements of loops that are close to the transmembrane interface in the receptors and changes in the intermonomer contacts, which alter the pentamer stability drastically. Structural, pharmacological and mutational analysis of both AChBPs shows how 3 amino acid changes in the binding site contribute to a 5-10-fold difference in affinity for nicotinic ligands. Comparison of these structures will be valuable for improving structure-function studies of ligand-gated ion channel receptors, including signal transduction, homology modeling, and drug design.
About this StructureAbout this Structure
2BJ0 is a Single protein structure of sequence from Bulinus truncatus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of acetylcholine-binding protein from Bulinus truncatus reveals the conserved structural scaffold and sites of variation in nicotinic acetylcholine receptors., Celie PH, Klaassen RV, van Rossum-Fikkert SE, van Elk R, van Nierop P, Smit AB, Sixma TK, J Biol Chem. 2005 Jul 15;280(28):26457-66. Epub 2005 May 16. PMID:15899893
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