2bcj: Difference between revisions

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Revision as of 17:00, 20 March 2008

File:2bcj.gif

, resolution 3.061Å

Ligands:

, , and

Gene:

ADRBK1, GRK2 (Bos taurus), GNB1 (Bos taurus), GNG2 (Bos taurus), Gnaq (Rattus norvegicus,Mus musculus)

Activity:

[Beta-adrenergic-receptor_kinase [Beta-adrenergic-receptor] kinase], with EC number 2.7.11.15

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits

OverviewOverview

G protein-coupled receptor kinase 2 (GRK2) plays a key role in the desensitization of G protein-coupled receptor signaling by phosphorylating activated heptahelical receptors and by sequestering heterotrimeric G proteins. We report the atomic structure of GRK2 in complex with Galphaq and Gbetagamma, in which the activated Galpha subunit of Gq is fully dissociated from Gbetagamma and dramatically reoriented from its position in the inactive Galphabetagamma heterotrimer. Galphaq forms an effector-like interaction with the GRK2 regulator of G protein signaling (RGS) homology domain that is distinct from and does not overlap with that used to bind RGS proteins such as RGS4.

About this StructureAbout this Structure

2BCJ is a Protein complex structure of sequences from Bos taurus and Rattus norvegicus,mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex., Tesmer VM, Kawano T, Shankaranarayanan A, Kozasa T, Tesmer JJ, Science. 2005 Dec 9;310(5754):1686-90. PMID:16339447 [[Category: [Beta-adrenergic-receptor] kinase]]

Page seeded by OCA on Thu Mar 20 16:00:08 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2bcj.gif|left|200px]]<br /><applet load="2bcj" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2bcj.gif|left|200px]]
-caption="2bcj, resolution 3.061&Aring;" />
-'''Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits'''<br />
+ {{Structure
+|PDB= 2bcj |SIZE=350|CAPTION= <scene name='initialview01'>2bcj</scene>, resolution 3.061&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/[Beta-adrenergic-receptor]_kinase [Beta-adrenergic-receptor] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.15 2.7.11.15]
+|GENE= ADRBK1, GRK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), GNB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), GNG2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), Gnaq ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Rattus norvegicus,Mus musculus])
+}}
+'''Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-BCJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus,mus_musculus Rattus norvegicus,mus musculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ALF:'>ALF</scene>, <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/[Beta-adrenergic-receptor]_kinase [Beta-adrenergic-receptor] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.15 2.7.11.15] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BCJ OCA].
+BCJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Rattus_norvegicus,mus_musculus Rattus norvegicus,mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BCJ OCA].
 ==Reference==
-Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex., Tesmer VM, Kawano T, Shankaranarayanan A, Kozasa T, Tesmer JJ, Science. 2005 Dec 9;310(5754):1686-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16339447 16339447]
+Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex., Tesmer VM, Kawano T, Shankaranarayanan A, Kozasa T, Tesmer JJ, Science. 2005 Dec 9;310(5754):1686-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16339447 16339447]
 [[Category: Bos taurus]]
 [[Category: Protein complex]]
@@ Line 26: / Line 35: @@
 [[Category: wd40 protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:21 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:00:08 2008''