2b2g: Difference between revisions
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'''MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid''' | {{Structure | ||
|PDB= 2b2g |SIZE=350|CAPTION= <scene name='initialview01'>2b2g</scene>, resolution 3.02Å | |||
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'''MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2B2G is a [ | 2B2G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2G OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2., Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG, Structure. 2006 Mar;14(3):487-95. PMID:[http:// | Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2., Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG, Structure. 2006 Mar;14(3):487-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16531233 16531233] | ||
[[Category: Enterobacterio phage ms2]] | [[Category: Enterobacterio phage ms2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: virus/viral]] | [[Category: virus/viral]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:56:47 2008'' | ||
Revision as of 16:56, 20 March 2008
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MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid
OverviewOverview
Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
About this StructureAbout this Structure
2B2G is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2., Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG, Structure. 2006 Mar;14(3):487-95. PMID:16531233
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