4lw5: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{STRUCTURE_4lw5|  PDB=4lw5  |  SCENE=  }}
+==Crystal structure of all-trans green fluorescent protein==
-===Crystal structure of all-trans green fluorescent protein===
+<StructureSection load='4lw5' size='340' side='right' caption='[[4lw5]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
-{{ABSTRACT_PUBMED_24408076}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4lw5]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LW5 FirstGlance]. <br>
+</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lw5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lw5 RCSB], [http://www.ebi.ac.uk/pdbsum/4lw5 PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Wild-type green fluorescent protein (GFP) folds on a time scale of minutes. The slow step in folding is a cis-trans peptide bond isomerization. The only conserved cis-peptide bond in the native GFP structure, at P89, was remodeled by the insertion of two residues, followed by iterative energy minimization and side chain design. The engineered GFP was synthesized and found to fold faster and more efficiently than its template protein, recovering 50% more of its fluorescence upon refolding. The slow phase of folding is faster and smaller in amplitude, and hysteresis in refolding has been eliminated. The elimination of a previously reported kinetically trapped state in refolding suggests that X-P89 is trans in the trapped state. A 2.55 A resolution crystal structure revealed that the new variant contains only trans-peptide bonds, as designed. This is the first instance of a computationally remodeled fluorescent protein that folds faster and more efficiently than wild type.
-==Function==
+Green-lighting green fluorescent protein: Faster and more efficient folding by eliminating a cis-trans peptide isomerization event.,Rosenman DJ, Huang YM, Xia K, Fraser K, Jones VE, Lamberson CM, Van Roey P, Colon W, Bystroff C Protein Sci. 2014 Jan 9. doi: 10.1002/pro.2421. PMID:24408076<ref>PMID:24408076</ref>
-[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[4lw5]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW5 OCA].
+</div>
 ==See Also==
 *[[Green Fluorescent Protein|Green Fluorescent Protein]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:024408076</ref><references group="xtra"/><references/>
+__TOC__
-[[Category: Bystroff, C.]]
+</StructureSection>
-[[Category: Colon, W.]]
+[[Category: Aeqvi]]
-[[Category: Huang, Y M.]]
+[[Category: Bystroff, C]]
-[[Category: Rosenman, D J.]]
+[[Category: Colon, W]]
-[[Category: Vanroey, P.]]
+[[Category: Huang, Y M]]
-[[Category: Xia, K.]]
+[[Category: Rosenman, D J]]
+[[Category: Vanroey, P]]
+[[Category: Xia, K]]
 [[Category: 11-stranded beta barrel]]
 [[Category: Fluorescent protein]]