2azx: Difference between revisions
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[[Image:2azx.gif|left|200px]] | [[Image:2azx.gif|left|200px]] | ||
'''Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase''' | {{Structure | ||
|PDB= 2azx |SIZE=350|CAPTION= <scene name='initialview01'>2azx</scene>, resolution 2.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Tryptophan--tRNA_ligase Tryptophan--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.2 6.1.1.2] | |||
|GENE= WARS, WRS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AZX is a [ | 2AZX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZX OCA]. | ||
==Reference== | ==Reference== | ||
Two conformations of a crystalline human tRNA synthetase-tRNA complex: implications for protein synthesis., Yang XL, Otero FJ, Ewalt KL, Liu J, Swairjo MA, Kohrer C, RajBhandary UL, Skene RJ, McRee DE, Schimmel P, EMBO J. 2006 Jun 21;25(12):2919-29. Epub 2006 May 25. PMID:[http:// | Two conformations of a crystalline human tRNA synthetase-tRNA complex: implications for protein synthesis., Yang XL, Otero FJ, Ewalt KL, Liu J, Swairjo MA, Kohrer C, RajBhandary UL, Skene RJ, McRee DE, Schimmel P, EMBO J. 2006 Jun 21;25(12):2919-29. Epub 2006 May 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16724112 16724112] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: TRP]] | [[Category: TRP]] | ||
[[Category: association complex and dissociation complex]] | [[Category: association complex and dissociation complex]] | ||
[[Category: two synthetase-trna complex with distinct | [[Category: two synthetase-trna complex with distinct conformation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:55:54 2008'' | ||
Revision as of 16:55, 20 March 2008
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| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | WARS, WRS (Homo sapiens) | ||||||
| Activity: | Tryptophan--tRNA ligase, with EC number 6.1.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Charged and uncharged tRNAs adopt distinct conformations when complexed with human tryptophanyl-tRNA synthetase
OverviewOverview
Aminoacylation of tRNA is the first step of protein synthesis. Here, we report the co-crystal structure of human tryptophanyl-tRNA synthetase and tRNATrp. This enzyme is reported to interact directly with elongation factor 1alpha, which carries charged tRNA to the ribosome. Crystals were generated from a 50/50% mixture of charged and uncharged tRNATrp. These crystals captured two conformations of the complex, which are nearly identical with respect to the protein and a bound tryptophan. They are distinguished by the way tRNA is bound. In one, uncharged tRNA is bound across the dimer, with anticodon and acceptor stem interacting with separate subunits. In this cross-dimer tRNA complex, the class I enzyme has a class II-like tRNA binding mode. This structure accounts for biochemical investigations of human TrpRS, including species-specific charging. In the other conformation, presumptive aminoacylated tRNA is bound only by the anticodon, the acceptor stem being free and having space to interact precisely with EF-1alpha, suggesting that the product of aminoacylation can be directly handed off to EF-1alpha for the next step of protein synthesis.
DiseaseDisease
Known disease associated with this structure: Wolcott-Rallison syndrome OMIM:[604032]
About this StructureAbout this Structure
2AZX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Two conformations of a crystalline human tRNA synthetase-tRNA complex: implications for protein synthesis., Yang XL, Otero FJ, Ewalt KL, Liu J, Swairjo MA, Kohrer C, RajBhandary UL, Skene RJ, McRee DE, Schimmel P, EMBO J. 2006 Jun 21;25(12):2919-29. Epub 2006 May 25. PMID:16724112
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