2aak: Difference between revisions
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==Overview== | ==Overview== | ||
The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme, (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been, determined with the use of multiple isomorphous replacement techniques and, refined at 2.4-A resolution by simulated annealing and restrained, least-squares. This E2 is an alpha/beta protein, with four alpha-helices, and a four-stranded antiparallel beta-sheet. The NH2 and COOH termini, which may be important for interaction with other enzymes and substrates, in the ubiquitin-conjugation pathway, are on the opposite side of the, molecule from the cysteine residue that binds to the COOH terminus of, ubiquitin. This structure should now allow for the rational analysis of E2, function by in vitro mutagenesis and facilitate the effective design . | The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme, (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been, determined with the use of multiple isomorphous replacement techniques and, refined at 2.4-A resolution by simulated annealing and restrained, least-squares. This E2 is an alpha/beta protein, with four alpha-helices, and a four-stranded antiparallel beta-sheet. The NH2 and COOH termini, which may be important for interaction with other enzymes and substrates, in the ubiquitin-conjugation pathway, are on the opposite side of the, molecule from the cysteine residue that binds to the COOH terminus of, ubiquitin. This structure should now allow for the rational analysis of E2, function by in vitro mutagenesis and facilitate the effective design of, E2s with unique specificities or catalytic functions. | ||
==About this Structure== | ==About this Structure== | ||
2AAK is a | 2AAK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. This structure superseeds the now removed PDB entry 1AAK. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Structure known Active Site: C88. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AAK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ubiquitin conjugation]] | [[Category: ubiquitin conjugation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:23:11 2007'' | ||